Muscares
Comparative genomics of Vitis vinifera and Muscadinia rotundifolia for the study of disease resistance in the Vitaceae
ANR funded project ANR-08-GENM-007, with the support of CNIV (Comité National des Interprofessions des Vins d'appellation d'origine)
Muscadinia rotundifolia is closely related to Vitis and it is a source of resistance to several pathogens that is currently being used as a genitor in breeding programmes at INRA.
The main goal of this project is to take advantage of the existence of the grapevine genome sequence to accelerate the functional characterization of the resistance to several grapevine pathogens found in M. rotundifolia. To achieve this goal, a step of development of resources for the analysis and exploitation of the syntheny between V. vinifera and M. rotundifolia is required.
The stages of the project are:
- Development of markers for genetic mapping in M. rotundifolia.
- Genetic mapping in M. rotundifolia and development of a comparative genomic map with V. vinifera. Comparing both genetic maps will unveil the degree of syntheny existing between both species and reveal the position of the clusters of resistance genes in M. rotundifolia.
- Study of microsyntheny between M. rotundifolia and V. vinifera in two regions involved into disease resistance and study of the mechanisms of introgression. This research will result in a better understanding of the suppression of genetic recombination found in some hybrids Vitis x Muscadinia.
- Constitution of a collection of M. rotundifolia NBS-LRR resistance genes and preliminary testing of candidate genes. Anchoring a M. rotundifolia BAC library to the grapevine reference genome sequence using the BES, assisted by comparative mapping, will result in the identification of M. rotundifolia BAC clones containing resistance genes. The sequencing of some of these BAC clones will allow the identification of candidate genes.
Duration: 01/01/2009 to 31/12/2011
Coordinator: Dr. Pedro F. Mestre
| Laboratories | Address | Person | Links |
|
UMR 1131 INRA / UDS Santé de la Vigne et Qualité du Vin 28, rue de Herrlisheim, BP 507 68021 Colmar cedex France | Coordinator Dr. Pedro F. Mestre | Santé de la Vigne et Qualité du Vin |
|
UMR INRA CNRS Université d’Evry Génomique Végétale F-91057 Evry cedex France | Dr. Anne-Françoise Adam-Blondon | Grapevine and Forest Trees Genomics |
|
URGI INRA de Versailles Route de Saint-Cyr F-78000 Versailles, France | Dr. Nathalie Choisne | Unité de Recherche en Génomique Info |
| EPGV |
EPGV INRA; CEA/ IG-CNG 2 rue Gaston Crémieux, CP 57021 F-91057 Evry Cedex, France | Dr. Dominique Brunel | Etude du Polymorphisme des Génomes Végétaux |
|
Plate-Forme de cytogénétique Moléculaire DGAP UMR 118 "Amélioration des Plantes et Biotechnologie Végétal" Domaine de la Motte, BP 35327 35653 Le Rheu cedex, France | Dr. Olivier Coriton | Plate-Forme de cytogénétique moléculaire |
Data
- Access to private data with GBrowse (grapevine genome 12X version with Muscares data)
- Dataset comprising the resequencing raw data
- Quality control & Cleaning process for Illumina GAII runs :
Tools
| URGI shared area | MUSCARES directory with Alfresco
|
To share documents with other MUSCARES project members, use Alfresco, our web software of electronic documents management .
To access to the Alfresco tool :
- the first form is the secured web server authentification with the URGI login/password.
- the 2nd form is Alfresco authentification with the Alfresco login/password.
The MUSCARES directory path is Compagny Home > Projects > Muscares.
To ask for an account on the URGI secure server, please complete the
form
To ask for an account on the Alfresco tool, please
contact us
Funding
Creation date: 01 Apr 2010
eZ PublishPublication supervisor: A-F. Adam-Blondon
Read Credits & General Terms of Use
Read How to cite