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 Fungal Genet Biol. 2005 Jan;42(1):30-41.

01 Jan 2005   Truncated and RIP-degenerated copies of the LTR retrotransposon /Pholy /are clustered in a pericentromeric region of the Leptosphaeria maculans genome

Attard A, Gout L, Ross S, Parlange F, Cattolico L, Balesdent MH, Rouxel T

The LMR1 5.2 kb interspersed repeat of Leptosphaeria maculans was described by Taylor and Borgmann [Mol. Plant Microbe Interact. 7 (1994) 181] as an uncharacterized repeated element sharing homologies with both LINEs and SINEs. Here, we used the LMR1 sequence as a template to identify the full-length element within a 184-kb genomic sequence corresponding to the pericentromeric region of the 2.80 Mb chromosome of isolate v23.1.3. This region comprises (i) one 6980-bp full-sized Pholy element bordered by two 275- to 280-bp long terminal repeats (LTRs), (ii) five Pholy-related sequences, usually truncated at their 3' ends, and (iii) five solo-LTRs. Structural features strongly suggested that Pholy corresponds to an ancient copia-like retrotransposon, sharing strong homologies with the Elsa retrotransposon of Stagonospora nodorum. Pholy was also suggested to be specific to pericentromeric regions. Comparative analysis of the structure of the Pholy-like sequences occurring in the 184-kb contig and in other parts of the genome showed that this family of repeats is highly degenerated following extensive repeat induced point mutation (RIP).

Keywords: Leptosphaeria maculans; LTR retrotransposon
Update: 08 Aug 2014
Creation date: 12 Apr 2010