Protein : Qrob_P0496040.2 Q. robur
Protein Identifier  ? Qrob_P0496040.2 Organism . Name  Quercus robur
Score  70.1 Score Type  egn
Protein Description  (M=4) 5.4.99.28 - tRNA pseudouridine(32) synthase. Code Enzyme  EC:5.4.99.28
Gene Prediction Quality  validated Protein length 

Sequence

Length: 196  
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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0003723 RNA binding Interacting selectively and non-covalently with an RNA molecule or a portion thereof.
GO:0001522 pseudouridine synthesis The intramolecular conversion of uridine to pseudouridine within an RNA molecule. This posttranscriptional base modification occurs in tRNA, rRNA, and snRNAs.
GO:0009451 RNA modification The covalent alteration of one or more nucleotides within an RNA molecule to produce an RNA molecule with a sequence that differs from that coded genetically.
GO:0009982 pseudouridine synthase activity Catalysis of the reaction: RNA uridine = RNA pseudouridine. Conversion of uridine in an RNA molecule to pseudouridine by rotation of the C1'-N-1 glycosidic bond of uridine in RNA to a C1'-C5.

38 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|gmx:100811500 2 195 + 194 Gaps:22 46.19 381 63.64 4e-60 RNA pseudourine synthase 7-like
blastp_kegg lcl|mtr:MTR_3g098940 2 195 + 194 Gaps:22 46.32 380 60.80 3e-57 RNA pseudourine synthase
blastp_kegg lcl|rcu:RCOM_1504920 54 195 + 142 Gaps:6 37.40 385 70.83 1e-56 ribosomal pseudouridine synthase putative (EC:3.2.1.17)
blastp_kegg lcl|pvu:PHAVU_009G162600g 2 195 + 194 Gaps:23 46.17 379 61.14 3e-56 hypothetical protein
blastp_kegg lcl|fve:101293481 54 195 + 142 Gaps:3 37.86 383 66.21 2e-55 RNA pseudourine synthase 7-like
blastp_kegg lcl|cmo:103502297 2 195 + 194 Gaps:22 46.43 392 58.24 2e-55 RNA pseudouridine synthase 7
blastp_kegg lcl|sot:102599479 54 195 + 142 Gaps:5 37.66 385 66.90 1e-54 RNA pseudouridine synthase 7-like
blastp_kegg lcl|sly:101254284 54 195 + 142 Gaps:5 37.86 383 66.21 2e-54 RNA pseudourine synthase 7-like
blastp_kegg lcl|cam:101510279 2 195 + 194 Gaps:22 46.32 380 57.95 2e-53 RNA pseudourine synthase 7-like
blastp_kegg lcl|csv:101221837 2 195 + 194 Gaps:21 46.06 393 56.35 3e-52 RNA pseudourine synthase 7-like
blastp_pdb 1v9k_B 86 135 + 50 none 21.93 228 52.00 1e-09 mol:protein length:228 Ribosomal large subunit pseudouridine synthas
blastp_pdb 1v9k_A 86 135 + 50 none 21.93 228 52.00 1e-09 mol:protein length:228 Ribosomal large subunit pseudouridine synthas
blastp_pdb 1xpi_B 86 135 + 50 none 21.65 231 52.00 1e-09 mol:protein length:231 Ribosomal large subunit pseudouridine synthas
blastp_pdb 1xpi_A 86 135 + 50 none 21.65 231 52.00 1e-09 mol:protein length:231 Ribosomal large subunit pseudouridine synthas
blastp_pdb 2i82_D 87 137 + 51 Gaps:3 23.96 217 48.08 7e-07 mol:protein length:217 Ribosomal large subunit pseudouridine synthas
blastp_pdb 2i82_C 87 137 + 51 Gaps:3 23.96 217 48.08 7e-07 mol:protein length:217 Ribosomal large subunit pseudouridine synthas
blastp_pdb 2i82_B 87 137 + 51 Gaps:3 23.96 217 48.08 7e-07 mol:protein length:217 Ribosomal large subunit pseudouridine synthas
blastp_pdb 2i82_A 87 137 + 51 Gaps:3 23.96 217 48.08 7e-07 mol:protein length:217 Ribosomal large subunit pseudouridine synthas
blastp_uniprot_sprot sp|Q9LU60|PUS7_ARATH 54 195 + 142 Gaps:13 34.68 395 59.12 5e-43 RNA pseudouridine synthase 7 OS Arabidopsis thaliana GN At5g51140 PE 2 SV 1
blastp_uniprot_sprot sp|Q0E0Y3|PUS7_ORYSJ 2 195 + 194 Gaps:25 47.07 393 46.49 1e-39 RNA pseudouridine synthase 7 OS Oryza sativa subsp. japonica GN Os02g0512300 PE 2 SV 2
blastp_uniprot_sprot sp|Q09709|YA32_SCHPO 87 146 + 60 none 15.23 394 61.67 9e-17 Uncharacterized protein C18B11.02c OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN SPAC18B11.02c PE 3 SV 1
blastp_uniprot_sprot sp|Q12069|PUS9_YEAST 87 151 + 65 Gaps:1 14.29 462 59.09 8e-16 tRNA pseudouridine(32) synthase mitochondrial OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN PUS9 PE 1 SV 1
blastp_uniprot_sprot sp|Q12362|RIB2_YEAST 87 146 + 60 none 10.15 591 56.67 1e-13 Bifunctional protein RIB2 OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN RIB2 PE 1 SV 1
blastp_uniprot_sprot sp|Q8IZ73|RUSD2_HUMAN 86 135 + 50 none 9.17 545 58.00 1e-12 RNA pseudouridylate synthase domain-containing protein 2 OS Homo sapiens GN RPUSD2 PE 1 SV 2
blastp_uniprot_sprot sp|Q149F1|RUSD2_MOUSE 86 135 + 50 none 9.04 553 58.00 2e-12 RNA pseudouridylate synthase domain-containing protein 2 OS Mus musculus GN Rpusd2 PE 2 SV 2
blastp_uniprot_sprot sp|P75485|Y292_MYCPN 70 135 + 66 none 21.36 309 45.45 1e-11 Uncharacterized RNA pseudouridine synthase MG209 homolog OS Mycoplasma pneumoniae (strain ATCC 29342 / M129) GN MPN_292 PE 3 SV 1
blastp_uniprot_sprot sp|O16686|YK27_CAEEL 87 137 + 51 none 11.81 432 54.90 5e-10 Uncharacterized protein K07E8.7 OS Caenorhabditis elegans GN K07E8.7 PE 3 SV 1
blastp_uniprot_sprot sp|Q87N15|RLUC_VIBPA 87 135 + 49 none 15.56 315 53.06 5e-09 Ribosomal large subunit pseudouridine synthase C OS Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) GN rluC PE 3 SV 1
rpsblast_cdd gnl|CDD|211331 53 138 + 86 Gaps:7 38.97 213 48.19 6e-24 cd02557 PseudoU_synth_ScRIB2 Pseudouridine synthases similar to Saccharomyces cerevisiae RIB2. Pseudouridine synthase Saccharomyces cerevisiae RIB2_like. This group is comprised of eukaryotic and bacterial proteins similar to Saccharomyces cerevisiae RIB2 S. cerevisiae Pus6p and human hRPUDSD2. S. cerevisiae RIB2 displays two distinct catalytic activities. The N-terminal domain of RIB2 is RNA:psi-synthase which makes psi32 on cytoplasmic tRNAs. Psi32 is highly phylogenetically conserved. The C-terminal domain of RIB2 has a DRAP deaminase activity which catalyses the formation of 5-amino-6-ribitylamino-2 4(1H 3H)-pyrimidinedione 5'-phosphate from 2 5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate during riboflavin biosynthesis. S. cerevisiae Pus6p makes the psi31 of cytoplasmic and mitochondrial tRNAs.
rpsblast_cdd gnl|CDD|161659 86 135 + 50 none 16.72 299 60.00 2e-18 TIGR00005 rluA_subfam pseudouridine synthase RluA family. In E. coli RluD (SfhB) modifies uridine to pseudouridine at 23S RNA U1911 1915 and 1917 RluC modifies 955 2504 and 2580 and RluA modifies U746 and tRNA U32. An additional homolog from E. coli outside this family TruC modifies uracil-65 in transfer RNAs to pseudouridine.

4 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 53 193 141 PTHR10436:SF87 none none none
SUPERFAMILY 84 141 58 SSF55120 none none IPR020103
PANTHER 53 193 141 PTHR10436 none none none
Pfam 85 123 39 PF00849 none RNA pseudouridylate synthase IPR006145

0 Localization

0 Qtllist

0 Targeting