Protein : Qrob_P0496030.2 Q. robur
Protein Identifier  ? Qrob_P0496030.2 Organism . Name  Quercus robur
Score  72.0 Score Type  egn
Protein Description  (M=4) 5.4.99.28 - tRNA pseudouridine(32) synthase. Code Enzyme  EC:5.4.99.28
Gene Prediction Quality  validated Protein length 

Sequence

Length: 203  
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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0003723 RNA binding Interacting selectively and non-covalently with an RNA molecule or a portion thereof.
GO:0001522 pseudouridine synthesis The intramolecular conversion of uridine to pseudouridine within an RNA molecule. This posttranscriptional base modification occurs in tRNA, rRNA, and snRNAs.
GO:0009451 RNA modification The covalent alteration of one or more nucleotides within an RNA molecule to produce an RNA molecule with a sequence that differs from that coded genetically.
GO:0009982 pseudouridine synthase activity Catalysis of the reaction: RNA uridine = RNA pseudouridine. Conversion of uridine in an RNA molecule to pseudouridine by rotation of the C1'-N-1 glycosidic bond of uridine in RNA to a C1'-C5.

27 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|rcu:RCOM_1504920 3 196 + 194 Gaps:39 56.36 385 69.59 1e-95 ribosomal pseudouridine synthase putative (EC:3.2.1.17)
blastp_kegg lcl|cam:101510279 1 191 + 191 Gaps:41 55.79 380 70.28 5e-95 RNA pseudourine synthase 7-like
blastp_kegg lcl|cmo:103502297 2 193 + 192 Gaps:37 55.36 392 68.20 8e-95 RNA pseudouridine synthase 7
blastp_kegg lcl|mtr:MTR_3g098940 1 191 + 191 Gaps:41 55.79 380 69.81 2e-93 RNA pseudourine synthase
blastp_kegg lcl|vvi:100255954 3 191 + 189 Gaps:41 54.40 386 70.95 3e-93 RNA pseudourine synthase 7-like
blastp_kegg lcl|pvu:PHAVU_009G162600g 3 191 + 189 Gaps:41 55.41 379 70.00 4e-93 hypothetical protein
blastp_kegg lcl|gmx:100811500 3 191 + 189 Gaps:40 55.38 381 70.14 8e-93 RNA pseudourine synthase 7-like
blastp_kegg lcl|pop:POPTR_0015s11340g 3 196 + 194 Gaps:36 56.70 388 65.91 2e-92 POPTRDRAFT_776329 hypothetical protein
blastp_kegg lcl|csv:101221837 2 193 + 192 Gaps:35 55.73 393 66.21 5e-92 RNA pseudourine synthase 7-like
blastp_kegg lcl|fve:101293481 20 193 + 174 Gaps:31 53.52 383 67.32 3e-91 RNA pseudourine synthase 7-like
blastp_uniprot_sprot sp|Q0E0Y3|PUS7_ORYSJ 24 191 + 168 Gaps:31 50.64 393 63.82 2e-84 RNA pseudouridine synthase 7 OS Oryza sativa subsp. japonica GN Os02g0512300 PE 2 SV 2
blastp_uniprot_sprot sp|Q9LU60|PUS7_ARATH 3 191 + 189 Gaps:41 58.23 395 58.70 2e-79 RNA pseudouridine synthase 7 OS Arabidopsis thaliana GN At5g51140 PE 2 SV 1
blastp_uniprot_sprot sp|Q8IZ73|RUSD2_HUMAN 38 191 + 154 Gaps:32 34.13 545 33.33 1e-28 RNA pseudouridylate synthase domain-containing protein 2 OS Homo sapiens GN RPUSD2 PE 1 SV 2
blastp_uniprot_sprot sp|Q149F1|RUSD2_MOUSE 38 191 + 154 Gaps:32 33.63 553 33.33 1e-28 RNA pseudouridylate synthase domain-containing protein 2 OS Mus musculus GN Rpusd2 PE 2 SV 2
blastp_uniprot_sprot sp|O16686|YK27_CAEEL 43 193 + 151 Gaps:11 37.50 432 37.65 7e-28 Uncharacterized protein K07E8.7 OS Caenorhabditis elegans GN K07E8.7 PE 3 SV 1
blastp_uniprot_sprot sp|Q12069|PUS9_YEAST 60 191 + 132 Gaps:4 29.44 462 37.50 8e-27 tRNA pseudouridine(32) synthase mitochondrial OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN PUS9 PE 1 SV 1
blastp_uniprot_sprot sp|Q09709|YA32_SCHPO 31 193 + 163 Gaps:32 49.49 394 29.74 9e-25 Uncharacterized protein C18B11.02c OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN SPAC18B11.02c PE 3 SV 1
blastp_uniprot_sprot sp|Q12362|RIB2_YEAST 64 188 + 125 Gaps:4 21.83 591 37.98 1e-22 Bifunctional protein RIB2 OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN RIB2 PE 1 SV 1
blastp_uniprot_sprot sp|P53294|PUS6_YEAST 41 191 + 151 Gaps:50 49.75 404 28.86 1e-17 tRNA pseudouridine(31) synthase OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN PUS6 PE 1 SV 1
blastp_uniprot_sprot sp|P72970|Y1592_SYNY3 61 185 + 125 Gaps:6 44.33 291 28.68 5e-09 Uncharacterized RNA pseudouridine synthase slr1592 OS Synechocystis sp. (strain PCC 6803 / Kazusa) GN slr1592 PE 3 SV 1
rpsblast_cdd gnl|CDD|211331 86 191 + 106 Gaps:5 52.11 213 44.14 8e-37 cd02557 PseudoU_synth_ScRIB2 Pseudouridine synthases similar to Saccharomyces cerevisiae RIB2. Pseudouridine synthase Saccharomyces cerevisiae RIB2_like. This group is comprised of eukaryotic and bacterial proteins similar to Saccharomyces cerevisiae RIB2 S. cerevisiae Pus6p and human hRPUDSD2. S. cerevisiae RIB2 displays two distinct catalytic activities. The N-terminal domain of RIB2 is RNA:psi-synthase which makes psi32 on cytoplasmic tRNAs. Psi32 is highly phylogenetically conserved. The C-terminal domain of RIB2 has a DRAP deaminase activity which catalyses the formation of 5-amino-6-ribitylamino-2 4(1H 3H)-pyrimidinedione 5'-phosphate from 2 5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate during riboflavin biosynthesis. S. cerevisiae Pus6p makes the psi31 of cytoplasmic and mitochondrial tRNAs.
rpsblast_cdd gnl|CDD|30910 60 193 + 134 Gaps:11 50.17 289 30.34 7e-17 COG0564 RluA Pseudouridylate synthases 23S RNA-specific [Translation ribosomal structure and biogenesis].
rpsblast_cdd gnl|CDD|161659 48 188 + 141 Gaps:19 49.50 299 27.03 1e-16 TIGR00005 rluA_subfam pseudouridine synthase RluA family. In E. coli RluD (SfhB) modifies uridine to pseudouridine at 23S RNA U1911 1915 and 1917 RluC modifies 955 2504 and 2580 and RluA modifies U746 and tRNA U32. An additional homolog from E. coli outside this family TruC modifies uracil-65 in transfer RNAs to pseudouridine.
rpsblast_cdd gnl|CDD|211346 115 193 + 79 Gaps:9 47.57 185 36.36 3e-12 cd02869 PseudoU_synth_RluCD_like Pseudouridine synthase RsuA/RluD family. This group is comprised of eukaryotic bacterial and archeal proteins similar to eight site specific Escherichia coli pseudouridine synthases: RsuA RluA RluB RluC RluD RluE RluF and TruA. Pseudouridine synthases catalyze the isomerization of specific uridines in a n RNA molecule to pseudouridines (5-ribosyluracil psi) requiring no cofactors. E. coli RluC for example makes psi955 2504 and 2580 in 23S RNA. Some psi sites such as psi1917 in 23S RNA made by RluD are universally conserved. Other psi sites occur in a more restricted fashion for example psi2819 in 21S mitochondrial ribosomal RNA made by S. cerevisiae Pus5p is only found in mitochondrial large subunit rRNAs from some other species and in gram negative bacteria. The E. coli counterpart of this psi residue is psi2580 in 23S rRNA. psi2604in 23S RNA made by RluF has only been detected in E.coli.
rpsblast_cdd gnl|CDD|201472 110 195 + 86 Gaps:6 61.74 149 31.52 2e-10 pfam00849 PseudoU_synth_2 RNA pseudouridylate synthase. Members of this family are involved in modifying bases in RNA molecules. They carry out the conversion of uracil bases to pseudouridine. This family includes RluD a pseudouridylate synthase that converts specific uracils to pseudouridine in 23S rRNA. RluA from E. coli converts bases in both rRNA and tRNA.
rpsblast_cdd gnl|CDD|211332 69 181 + 113 Gaps:11 47.97 246 26.27 1e-09 cd02558 PSRA_1 Pseudouridine synthase a subgroup of the RluA family. This group is comprised of bacterial proteins assigned to the RluA family of pseudouridine synthases. Pseudouridine synthases catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil psi). No cofactors are required. The RluA family is comprised of proteins related to Escherichia coli RluA.
rpsblast_kog gnl|CDD|37130 36 194 + 159 Gaps:32 51.48 371 37.70 7e-38 KOG1919 KOG1919 KOG1919 RNA pseudouridylate synthases [RNA processing and modification].

5 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
SUPERFAMILY 93 192 100 SSF55120 none none IPR020103
PANTHER 1 191 191 PTHR10436:SF87 none none none
PANTHER 1 191 191 PTHR10436 none none none
Pfam 109 184 76 PF00849 none RNA pseudouridylate synthase IPR006145
ProSitePatterns 143 157 15 PS01129 none Rlu family of pseudouridine synthase signature. IPR006224

0 Localization

0 Qtllist

0 Targeting