GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P161670.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	bfu:BC1G_11806	1	421	421	n/a	82.71	509	96.20	0.24	0.0	L-lactate ferricytochrome c oxidoreductase
	ssl:SS1G_04765	1	421	421	n/a	82.71	509	89.31	4.99	0.0	hypothetical protein
	fgr:FG01027.1	1	421	421	Gaps:23	81.97	488	68.25	12.50	1e-160	hypothetical protein
	pno:SNOG_01213	1	434	434	Gaps:18	85.08	496	65.40	12.80	1e-155	hypothetical protein
	afv:AFLA_022200	1	421	421	Gaps:15	83.00	494	64.88	12.93	1e-154	mitochondrial cytochrome b2 putative
	aor:AO090012000974	1	421	421	Gaps:15	82.33	498	64.63	12.93	1e-153	glycolate oxidase
	mgr:MGG_00335	5	421	417	Gaps:18	78.91	531	64.44	12.17	1e-151	MG00335.4 hypothetical protein
	nfi:NFIA_017510	1	421	421	Gaps:14	82.29	497	64.06	13.94	1e-149	mitochondrial cytochrome b2 putative
	afm:AFUA_1G07200	1	421	421	Gaps:14	86.84	471	64.06	14.18	1e-149	mitochondrial cytochrome b2 (EC:1.1.2.3) K00101 L-lactate dehydrogenase (cytochrome) [EC:1.1.2.3]
	pcs:Pc22g11470	1	422	422	Gaps:35	82.67	502	63.86	12.29	1e-148	Pc22g11470
blastp_uniprot_sprot	sp\|P09437\|CYB2_HANAN	10	421	412	Gaps:27	67.89	573	36.50	20.05	1e-60	Cytochrome b2 mitochondrial OS Hansenula anomala GN CYB2 PE 1 SV 2
	sp\|P00175\|CYB2_YEAST	8	421	414	Gaps:28	68.36	591	36.39	19.06	3e-60	Cytochrome b2 mitochondrial OS Saccharomyces cerevisiae GN CYB2 PE 1 SV 1
	sp\|P05414\|GOX_SPIOL	119	421	303	Gaps:14	78.86	369	38.14	19.24	6e-44	Peroxisomal (S)-2-hydroxy-acid oxidase OS Spinacia oleracea PE 1 SV 1
	sp\|Q9NYQ2\|HAOX2_MOUSE	118	421	304	Gaps:18	81.02	353	39.51	20.28	2e-42	Hydroxyacid oxidase 2 OS Mus musculus GN Hao2 PE 2 SV 1
	sp\|Q9LRR9\|GOX2_ARATH	119	421	303	Gaps:14	79.29	367	37.11	20.62	3e-42	Probable peroxisomal (S)-2-hydroxy-acid oxidase 2 OS Arabidopsis thaliana GN At3g14420 PE 1 SV 1
	sp\|Q9LRS0\|GOX1_ARATH	119	421	303	Gaps:14	79.29	367	36.08	20.62	9e-41	Probable peroxisomal (S)-2-hydroxy-acid oxidase 1 OS Arabidopsis thaliana GN At3g14415 PE 1 SV 1
	sp\|Q07523\|HAOX2_RAT	119	421	303	Gaps:20	80.74	353	37.54	20.70	2e-39	Hydroxyacid oxidase 2 OS Rattus norvegicus GN Hao2 PE 1 SV 2
	sp\|Q9NYQ3\|HAOX2_HUMAN	118	421	304	Gaps:20	80.91	351	35.56	20.07	9e-39	Hydroxyacid oxidase 2 OS Homo sapiens GN HAO2 PE 2 SV 1
	sp\|Q54E41\|HAOX_DICDI	116	429	314	Gaps:17	77.06	388	35.45	19.40	6e-38	Hydroxyacid oxidase OS Dictyostelium discoideum GN haox PE 3 SV 1
	sp\|Q3ZBW2\|HAOX2_BOVIN	119	421	303	Gaps:22	80.74	353	35.44	20.70	1e-37	Hydroxyacid oxidase 2 OS Bos taurus GN HAO2 PE 2 SV 1
blastp_pdb	1sze_B	8	421	414	Gaps:28	79.06	511	36.39	19.06	5e-61	mol:protein length:511 Cytochrome b2 mitochondrial
	1sze_A	8	421	414	Gaps:28	79.06	511	36.39	19.06	5e-61	mol:protein length:511 Cytochrome b2 mitochondrial
	1ltd_B	8	421	414	Gaps:28	79.84	506	36.39	19.06	5e-61	mol:protein length:506 FLAVOCYTOCHROME B2
	1ltd_A	8	421	414	Gaps:28	79.84	506	36.39	19.06	5e-61	mol:protein length:506 FLAVOCYTOCHROME B2
	1kbi_B	8	421	414	Gaps:28	79.06	511	36.39	19.06	6e-61	mol:protein length:511 CYTOCHROME B2
	1kbi_A	8	421	414	Gaps:28	79.06	511	36.39	19.06	6e-61	mol:protein length:511 CYTOCHROME B2
	1fcb_B	8	421	414	Gaps:28	79.06	511	36.39	19.06	6e-61	mol:protein length:511 FLAVOCYTOCHROME B2
	1fcb_A	8	421	414	Gaps:28	79.06	511	36.39	19.06	6e-61	mol:protein length:511 FLAVOCYTOCHROME B2
	1szg_B	8	421	414	Gaps:28	79.06	511	36.14	19.06	1e-60	mol:protein length:511 Cytochrome b2 mitochondrial
	1szg_A	8	421	414	Gaps:28	79.06	511	36.14	19.06	1e-60	mol:protein length:511 Cytochrome b2 mitochondrial
rpsblast_cdd	gnl\|CDD\|73376	123	421	299	Gaps:14	82.85	344	50.88	14.04	1e-101	cd02922 FCB2_FMN Flavocytochrome b2 (FCB2) FMN-binding domain. FCB2 (AKA L-lactate:cytochrome c oxidoreductase) is a respiratory enzyme located in the intermembrane space of fungal mitochondria which catalyzes the oxidation of L-lactate to pyruvate. FCB2 also participates in a short electron-transport chain involving cytochrome c and cytochrome oxidase which ultimately directs the reducing equivalents gained from L-lactate oxidation to oxygen yielding one molecule of ATP for every L-lactate molecule consumed. FCB2 is composed of 2 domains: a C-terminal flavin-binding domain which includes the active site for lacate oxidation and an N-terminal b2-cytochrome domain required for efficient cytochrome c reduction. FCB2 is a homotetramer and contains two noncovalently bound cofactors FMN and heme per subunit..
	gnl\|CDD\|144604	131	421	291	Gaps:54	78.74	301	43.88	16.03	3e-72	pfam01070 FMN_dh FMN-dependent dehydrogenase.
	gnl\|CDD\|73371	123	421	299	Gaps:58	80.60	299	41.91	17.43	5e-56	cd02809 alpha_hydroxyacid_oxid_FMN Family of homologous FMN-dependent alpha-hydroxyacid oxidizing enzymes. This family occurs in both prokaryotes and eukaryotes. Members of this family include flavocytochrome b2 (FCB2) glycolate oxidase (GOX) lactate monooxygenase (LMO) mandelate dehydrogenase (MDH) and long chain hydroxyacid oxidase (LCHAO). In green plants glycolate oxidase is one of the key enzymes in photorespiration where it oxidizes glycolate to glyoxylate. LMO catalyzes the oxidation of L-lactate to acetate and carbon dioxide. MDH oxidizes (S)-mandelate to phenylglyoxalate. It is an enzyme in the mandelate pathway that occurs in several strains of Pseudomonas which converts (R)-mandelate to benzoate..
	gnl\|CDD\|166176	120	421	302	Gaps:19	78.85	364	38.33	19.16	8e-47	PLN02535 PLN02535 glycolate oxidase.
	gnl\|CDD\|166134	119	421	303	Gaps:14	79.29	367	36.77	20.62	3e-43	PLN02493 PLN02493 glycolate oxidase.
	gnl\|CDD\|73399	118	421	304	Gaps:17	81.77	351	32.06	19.86	6e-43	cd04737 LOX_like_FMN L-Lactate oxidase (LOX) FMN-binding domain. LOX is a member of the family of FMN-containing alpha-hydroxyacid oxidases and catalyzes the oxidation of l-lactate using molecular oxygen to generate pyruvate and H2O2. This family occurs in both prokaryotes and eukaryotes. Members of this family include flavocytochrome b2 (FCB2) glycolate oxidase (GOX) lactate monooxygenase (LMO) mandelate dehydrogenase (MDH) and long chain hydroxyacid oxidase (LCHAO)..
	gnl\|CDD\|31495	122	421	300	Gaps:19	79.72	360	33.10	16.38	8e-43	COG1304 LldD L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases [Energy production and conversion].
	gnl\|CDD\|73383	121	421	301	Gaps:28	79.11	383	29.04	17.49	3e-40	cd03332 LMO_FMN L-Lactate 2-monooxygenase (LMO) FMN-binding domain. LMO is a FMN-containing enzyme that catalyzes the conversion of L-lactate and oxygen to acetate carbon dioxide and water. LMO is a member of the family of alpha-hydroxy acid oxidases. It is thought to be a homooctamer with two- and four- fold axes in the center of the octamer..
	gnl\|CDD\|166620	163	421	259	Gaps:14	67.49	366	38.46	19.84	4e-37	PLN02979 PLN02979 glycolate oxidase.
	gnl\|CDD\|131755	119	419	301	Gaps:22	77.11	367	31.10	18.37	2e-33	TIGR02708 L_lactate_ox L-lactate oxidase. Members of this protein oxidize L-lactate to pyruvate reducing molecular oxygen to hydrogen peroxide. The enzyme is known in Aerococcus viridans Streptococcus iniae and some strains of Streptococcus pyogenes where it appears to contribute to virulence.
rpsblast_kog	gnl\|CDD\|35758	119	421	303	Gaps:13	80.44	363	39.38	19.18	2e-66	KOG0538 KOG0538 KOG0538 Glycolate oxidase [Energy production and conversion].
	gnl\|CDD\|35757	1	91	91	Gaps:2	71.77	124	38.20	12.36	2e-15	KOG0537 KOG0537 KOG0537 Cytochrome b5 [Energy production and conversion].
	gnl\|CDD\|35756	9	61	53	Gaps:1	35.86	145	42.31	15.38	3e-11	KOG0536 KOG0536 KOG0536 Flavohemoprotein b5+b5R [Energy production and conversion].
	gnl\|CDD\|39776	2	81	80	Gaps:2	47.90	167	37.50	17.50	8e-11	KOG4576 KOG4576 KOG4576 Sulfite oxidase heme-binding component [Energy production and conversion].
	gnl\|CDD\|39433	3	62	60	Gaps:2	13.49	430	37.93	18.97	1e-08	KOG4232 KOG4232 KOG4232 Delta 6-fatty acid desaturase/delta-8 sphingolipid desaturase [Lipid transport and metabolism].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	no results
sim4_Bot_allest	YCIA_533995	378861	379486	483	96.89	100.00	n/a	Botrytis cinerea HortResearch libraries
	B5BC_116_305_D09	378037	378841	806	97.52	100.00	n/a	B0510 In planta material
	PD0ABA15YC20FM1	378108	378799	692	100.00	100.00	n/a	BT4 young mycelium library
	PD0ADA5YI04CM1	377982	378612	631	99.37	100.00	n/a	B0510 mycelium - carbon sources library
	AL116091	377977	378430	580	99.34	78.28	n/a	BT4 Mycelium - nitrate starvation library
	PD0AEA3YK18CM1	377977	378509	637	99.44	83.67	n/a	BT4 appressoria on teflon library
	PD0AEA4YC12CM1	377977	378418	717	99.32	61.65	n/a	BT4 appressoria on teflon library
	YCMA_552756	378835	379654	676	99.70	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCHA_538353	378861	379639	635	99.69	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCGA_536884	379019	379696	534	99.63	100.00	n/a	Botrytis cinerea HortResearch libraries
sim4_Bot_allest4_clctg	SCL_bt4ctg_2254_bt4_SupSuperContig_246_31_4_1_bt4ctg_2255.Contig1_v5	377977	378841	1140	98.96	75.88	n/a	all_lib_clone_nr:7
sim4_Ss_allest	no results
blastx_uniprot_sprot	Q9NYQ3	377995	378999	351	37.14	89.74	n/a	Hydroxyacid oxidase 2 - Homo sapiens (Human)
	P09437	377983	379326	573	38.59	74.17	n/a	Cytochrome b2 mitochondrial precursor - Hansenula anomala (Yeast) (Candida pelliculosa)
	P00175	377983	379323	591	37.53	73.94	n/a	Cytochrome b2 mitochondrial precursor - Saccharomyces cerevisiae (Baker's yeast)
	P05414	377977	378996	369	39.02	88.89	n/a	Peroxisomal - Spinacia oleracea (Spinach)
	Q9LRR9	377977	378996	367	37.50	89.37	n/a	Probable peroxisomal - Arabidopsis thaliana (Mouse-ear cress)
	Q9NYQ2	377983	378999	353	39.56	90.93	n/a	Hydroxyacid oxidase 2 - Mus musculus (Mouse)
	Q9LRS0	377977	378996	367	36.59	89.37	n/a	Probable peroxisomal - Arabidopsis thaliana (Mouse-ear cress)
	Q07523	377983	378996	353	38.12	90.65	n/a	Hydroxyacid oxidase 2 - Rattus norvegicus (Rat)
	Q3ZBW2	377983	378996	353	37.50	90.65	n/a	Hydroxyacid oxidase 2 - Bos taurus (Bovine)
	Q9NYQ3	377995	378999	351	37.14	89.74	n/a	Hydroxyacid oxidase 2 - Homo sapiens (Human)
	Q9UJM8	377983	378996	370	36.36	89.19	n/a	Hydroxyacid oxidase 1 - Homo sapiens (Human)
blastx_uniprot_trembl	Q0V451	377983	379323	496	69.34	88.10	n/a	Putative uncharacterized protein - Phaeosphaeria nodorum (Septoria nodorum)
	A4RFI8	377983	379323	531	66.59	84.56	n/a	Putative uncharacterized protein - Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea)
	Q2UBJ4	377983	379323	498	66.06	87.55	n/a	Glycolate oxidase - Aspergillus oryzae
	Q4WJ52	377983	379323	471	65.52	92.36	n/a	Mitochondrial cytochrome b2 putative - Aspergillus fumigatus (Sartorya fumigata)
	A1CQU5	377983	379323	495	66.51	87.47	n/a	Mitochondrial cytochrome b2 putative - Aspergillus clavatus
	A1D3Q7	377983	379323	497	65.52	87.53	n/a	Mitochondrial cytochrome b2 putative - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
	Q7S0C1	377983	379323	520	63.88	87.31	n/a	Putative uncharacterized protein - Neurospora crassa
	Q1E4N8	377983	379323	492	66.59	86.99	n/a	Putative uncharacterized protein - Coccidioides immitis
blastx_FNG30_prot	SS1G_04765.1	377983	379323	509	91.72	87.82	n/a	sclerotinia_sclerotiorum hypothetical protein translation 510 aa
	FG01027.1	377983	379323	488	70.19	87.30	n/a	fusarium_graminearum hypothetical protein 489 aa
	SNU01213.1	377983	379323	496	69.34	88.10	n/a	Stagonospora nodorum hypothetical protein translation
	MGG_00335.5	377983	379323	531	66.59	84.56	n/a	magnaporthe_grisea hypothetical protein translation 532 aa
	AO090012000974	377983	379323	498	66.06	87.55	n/a	Aspergillus oryzae glycolate oxidase
	gi:70991238:ref:XP_750468.1:	377983	379323	471	65.52	92.36	n/a	Aspergillus fumigatus Af293 mitochondrial cytochrome b2
	jgi:Triree:31356	377983	379323	487	67.76	87.27	n/a	Trichoderma reesei
	NCU07362.2	377983	379323	520	63.88	87.31	n/a	Neurospora crassa hypothetical protein translation 521 aa
	CIMG_02475.2	377983	379323	492	66.59	86.99	n/a	Coccidioides immitis hypothetical protein translation 493 aa
	CHG06000.1	377983	379320	509	64.30	85.85	n/a	Chaetomium globosum hypothetical protein translation 510 aa
sim4_B0510_genes	no results
sim4_SS_genes	SS1G_04765.1	377984	379311	1530	85.24	86.80	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.86	377942	379927	169497	99.40	n/a	n/a	n/a
blastn_SS_supercontig	SS_supercontig_1.5	378002	379326	2230026	84.38	n/a	n/a	n/a
blat_oligo	botrytisP00160365	379284	379487	60	n/a	n/a	n/a	array_gene:BofuT4_P161670_1 . max_over_threshold:41.03 . min_over_threshold:4.91 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00160366	379018	379077	60	n/a	n/a	n/a	array_gene:BofuT4_P161670_1 . max_over_threshold:40.5 . min_over_threshold:7.6 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00160367	378844	378903	60	n/a	n/a	n/a	array_gene:BofuT4_P161670_1 . max_over_threshold:38.02 . min_over_threshold:5.58 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00160368	378620	378679	60	n/a	n/a	n/a	array_gene:BofuT4_P161670_1 . max_over_threshold:72.82 . min_over_threshold:12.32 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00160370	378607	378666	60	n/a	n/a	n/a	array_gene:BofuT4_P161670_1 . max_over_threshold:50.63 . min_over_threshold:8.13 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00160371	378600	378659	60	n/a	n/a	n/a	array_gene:BofuT4_P161670_1 . max_over_threshold:65.74 . min_over_threshold:8.98 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00160372	378510	378569	60	n/a	n/a	n/a	array_gene:BofuT4_P161670_1 . max_over_threshold:55.19 . min_over_threshold:5.64 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00160373	378314	378373	60	n/a	n/a	n/a	array_gene:BofuT4_P161670_1 . max_over_threshold:83.96 . min_over_threshold:15.17 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00160374	378012	378118	60	n/a	n/a	n/a	array_gene:BofuT4_P161670_1 . max_over_threshold:88 . min_over_threshold:19.68 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:1

Current manual annotation

Ortholog