GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P008630.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	bfu:BC1G_03902	1	458	458	n/a	100.00	458	100.00	0.00	0.0	hypothetical protein K08764 sterol carrier protein 2 [EC:2.3.1.176]
	ssl:SS1G_10890	1	458	458	Gaps:25	100.00	433	95.84	2.54	0.0	hypothetical protein K08764 sterol carrier protein 2 [EC:2.3.1.176]
	ncr:NCU03372	1	458	458	n/a	100.00	458	79.48	9.39	0.0	nonspecific lipid-transfer protein mitochondrial precursor K08764 sterol carrier protein 2 [EC:2.3.1.176]
	act:ACLA_045690	4	458	455	n/a	99.34	458	79.56	8.13	0.0	sterol carrier protein putative K08764 sterol carrier protein 2 [EC:2.3.1.176]
	pcs:Pc22g17070	4	458	455	n/a	99.34	458	80.22	7.03	0.0	Pc22g17070 K08764 sterol carrier protein 2 [EC:2.3.1.176]
	afm:AFUA_4G06380	4	458	455	n/a	99.34	458	78.68	9.01	0.0	sterol carrier protein K08764 sterol carrier protein 2 [EC:2.3.1.176]
	nfi:NFIA_109770	4	458	455	n/a	99.34	458	78.24	9.01	0.0	sterol carrier protein putative K08764 sterol carrier protein 2 [EC:2.3.1.176]
	afv:AFLA_113580	4	458	455	n/a	99.34	458	78.90	7.91	0.0	sterol carrier protein putative K08764 sterol carrier protein 2 [EC:2.3.1.176]
	aor:AO090023000951	4	458	455	n/a	99.34	458	78.90	7.91	0.0	peroxisomal 3-ketoacyl-CoA-thiolase P-44/SCP2 K08764 sterol carrier protein 2 [EC:2.3.1.176]
	cim:CIMG_04177	8	458	451	n/a	98.47	458	79.16	8.20	0.0	hypothetical protein K08764 sterol carrier protein 2 [EC:2.3.1.176]
blastp_uniprot_sprot	sp\|Q07598\|NLTP_CHICK	10	419	410	Gaps:11	76.60	547	53.94	14.56	1e-119	Non-specific lipid-transfer protein (Fragment) OS Gallus gallus GN SCP2 PE 2 SV 1
	sp\|O62742\|NLTP_RABIT	10	396	387	Gaps:5	71.30	547	56.41	15.38	1e-119	Non-specific lipid-transfer protein OS Oryctolagus cuniculus GN SCP2 PE 1 SV 1
	sp\|P11915\|NLTP_RAT	10	417	408	Gaps:15	76.97	547	52.02	17.34	1e-118	Non-specific lipid-transfer protein OS Rattus norvegicus GN Scp2 PE 1 SV 3
	sp\|P32020\|NLTP_MOUSE	5	417	413	Gaps:15	77.88	547	52.58	15.26	1e-117	Non-specific lipid-transfer protein OS Mus musculus GN Scp2 PE 1 SV 3
	sp\|P22307\|NLTP_HUMAN	10	396	387	Gaps:5	71.30	547	55.90	15.64	1e-115	Non-specific lipid-transfer protein OS Homo sapiens GN SCP2 PE 1 SV 2
	sp\|P07857\|NLTP_BOVIN	10	396	387	Gaps:4	71.64	543	56.04	14.91	1e-115	Non-specific lipid-transfer protein OS Bos taurus GN SCP2 PE 1 SV 2
	sp\|O26884\|Y793_METTH	11	397	387	Gaps:37	98.69	383	35.19	18.25	1e-50	Uncharacterized protein MTH_793 OS Methanobacterium thermoautotrophicum GN MTH_793 PE 4 SV 1
	sp\|Q58944\|Y1549_METJA	11	386	376	Gaps:31	93.62	392	31.88	20.98	8e-41	Uncharacterized protein MJ1549 OS Methanocaldococcus jannaschii GN MJ1549 PE 4 SV 1
	sp\|Q43935\|CATF_ACIAD	75	353	279	Gaps:78	71.07	401	28.07	15.79	1e-08	Beta-ketoadipyl-CoA thiolase OS Acinetobacter sp. (strain ADP1) GN catF PE 3 SV 1
	sp\|Q43974\|PCAF_ACIAD	75	353	279	Gaps:78	71.07	401	28.07	15.79	1e-08	Beta-ketoadipyl-CoA thiolase OS Acinetobacter sp. (strain ADP1) GN pcaF PE 1 SV 1
blastp_pdb	1ulq_H	81	365	285	Gaps:70	72.57	401	27.84	14.43	7e-10	mol:protein length:401 putative acetyl-CoA acetyltransferase
	1ulq_G	81	365	285	Gaps:70	72.57	401	27.84	14.43	7e-10	mol:protein length:401 putative acetyl-CoA acetyltransferase
	1ulq_F	81	365	285	Gaps:70	72.57	401	27.84	14.43	7e-10	mol:protein length:401 putative acetyl-CoA acetyltransferase
	1ulq_E	81	365	285	Gaps:70	72.57	401	27.84	14.43	7e-10	mol:protein length:401 putative acetyl-CoA acetyltransferase
	1ulq_D	81	365	285	Gaps:70	72.57	401	27.84	14.43	7e-10	mol:protein length:401 putative acetyl-CoA acetyltransferase
	1ulq_C	81	365	285	Gaps:70	72.57	401	27.84	14.43	7e-10	mol:protein length:401 putative acetyl-CoA acetyltransferase
	1ulq_B	81	365	285	Gaps:70	72.57	401	27.84	14.43	7e-10	mol:protein length:401 putative acetyl-CoA acetyltransferase
	1ulq_A	81	365	285	Gaps:70	72.57	401	27.84	14.43	7e-10	mol:protein length:401 putative acetyl-CoA acetyltransferase
	2iik_B	77	365	289	Gaps:59	67.46	418	24.82	18.09	2e-08	mol:protein length:418 3-ketoacyl-CoA thiolase peroxisomal
	2iik_A	77	365	289	Gaps:59	67.46	418	24.82	18.09	2e-08	mol:protein length:418 3-ketoacyl-CoA thiolase peroxisomal
rpsblast_cdd	gnl\|CDD\|169317	10	398	389	Gaps:3	99.23	391	55.41	14.69	1e-162	PRK08256 PRK08256 lipid-transfer protein Provisional.
	gnl\|CDD\|29416	12	395	384	Gaps:15	100.00	375	36.80	15.73	1e-101	cd00829 SCP-x_thiolase Thiolase domain associated with sterol carrier protein (SCP)-x isoform and related proteins SCP-2 has multiple roles in intracellular lipid circulation and metabolism. The N-terminal presequence in the SCP-x isoform represents a peroxisomal 3-ketacyl-Coa thiolase specific for branched-chain acyl CoAs which is proteolytically cleaved from the sterol carrier protein..
	gnl\|CDD\|73239	13	395	383	Gaps:25	99.75	393	34.95	15.05	1e-81	cd00826 nondecarbox_cond_enzymes nondecarboxylating condensing enzymes In general thiolases catalyze the reversible thiolytic cleavage of 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA a 2-step reaction involving a covalent intermediate formed with a catalytic cysteine. There are 2 functional different classes: thiolase-I (3-ketoacyl-CoA thiolase) and thiolase-II (acetoacetyl-CoA thiolase). Thiolase-I can cleave longer fatty acid molecules and plays an important role in the beta-oxidative degradation of fatty acids. Thiolase-II has a high substrate specificity. Although it can cleave acetoacyl-CoA its main function is the synthesis of acetoacyl-CoA from two molecules of acetyl-CoA which gives it importance in several biosynthetic pathways..
	gnl\|CDD\|168372	10	398	389	Gaps:43	98.71	389	36.46	16.41	7e-74	PRK06064 PRK06064 acetyl-CoA acetyltransferase Provisional.
	gnl\|CDD\|30532	7	397	391	Gaps:41	99.49	392	29.49	15.38	3e-56	COG0183 PaaJ Acetyl-CoA acetyltransferase [Lipid metabolism].
	gnl\|CDD\|168430	11	385	375	Gaps:43	93.47	398	31.99	14.52	3e-48	PRK06157 PRK06157 acetyl-CoA acetyltransferase Validated.
	gnl\|CDD\|168367	7	394	388	Gaps:41	99.00	399	30.89	16.96	7e-48	PRK06059 PRK06059 lipid-transfer protein Provisional.
	gnl\|CDD\|168987	78	391	314	Gaps:40	77.63	389	35.10	14.90	1e-45	PRK07516 PRK07516 acetyl-CoA acetyltransferase Provisional.
	gnl\|CDD\|105770	11	397	387	Gaps:34	98.96	385	31.23	17.32	1e-42	PRK12578 PRK12578 acetyl-CoA acetyltransferase Provisional.
	gnl\|CDD\|168509	10	394	385	Gaps:38	97.30	408	26.95	14.86	1e-36	PRK06289 PRK06289 acetyl-CoA acetyltransferase Provisional.
rpsblast_kog	gnl\|CDD\|36620	10	396	387	Gaps:4	95.34	408	58.35	13.88	1e-138	KOG1406 KOG1406 KOG1406 Peroxisomal 3-ketoacyl-CoA-thiolase P-44/SCP2 [Lipid transport and metabolism].
rpsblast_kog	gnl\|CDD\|36604	6	364	359	Gaps:78	91.16	396	24.38	16.90	5e-09	KOG1390 KOG1390 KOG1390 Acetyl-CoA acetyltransferase [Lipid transport and metabolism].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	no results
sim4_Bot_allest	YCHA_538764	340120	340774	595	99.16	100.00	n/a	Botrytis cinerea HortResearch libraries
	B2BC_045_106_A03	340208	340888	620	99.35	100.00	n/a	B0510 Spores suspension on bean library
	BcA1_3752_A21	340243	341033	731	99.32	100.00	n/a	BC A1 mycelium
	YCMA_548732	340028	340647	560	99.46	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCMA_548501	340008	340524	517	99.81	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCIA_546809	340043	340702	601	98.50	100.00	n/a	Botrytis cinerea HortResearch libraries
	PD0ACA15YK05FM1	340039	340820	722	99.86	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA10YM18FM1	340039	340822	724	100.00	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA8YM16FM1	340045	340819	715	100.00	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA9YM16FM1	340045	340743	639	99.69	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA22YN21FM1	340039	340455	417	99.28	100.00	n/a	BT4 mycelium - pH stress library
	PD0AHA14YL11CM1	339966	340537	572	100.00	100.00	n/a	BT4 appressoria on teflon library
	PD0AHA17YC11CM1	340887	341522	636	99.69	100.00	n/a	BT4 appressoria on teflon library
sim4_Bot_allest4_clctg	CL_bt4_SuperContig_3_1_bt4ctg_0115_005.Contig1_v5	339966	341522	1497	99.87	100.00	n/a	all_lib_clone_nr:9
sim4_Ss_allest	G781P547FB8.T0	340826	341484	756	85.56	87.04	n/a	Ss ESTs grown as developing sclerotia
	G781P545FF11.T0	340863	341484	721	85.76	78.92	n/a	Ss ESTs grown as developing sclerotia
	G781P554FA2.T0	340922	341484	757	85.23	74.24	n/a	Ss ESTs grown as developing sclerotia
	G786P580RA8.T0	340076	340788	834	86.68	63.91	n/a	Ss ESTs grown as mycelia at neutral pH
	G786P554RD2.T0	340076	340792	782	85.97	77.49	n/a	Ss ESTs grown as mycelia at neutral pH
	G786P536RC1.T0	340076	340710	727	86.31	72.35	n/a	Ss ESTs grown as mycelia at neutral pH
	G781P541RG5.T0	340076	340698	848	86.63	60.85	n/a	Ss ESTs grown as developing sclerotia
	G781P545RF11.T0	340076	340644	775	84.44	66.32	n/a	Ss ESTs grown as developing sclerotia
	G781P547RB8.T0	340076	340689	688	84.92	73.26	n/a	Ss ESTs grown as developing sclerotia
	G781P541FG5.T0	340884	341484	760	84.13	79.61	n/a	Ss ESTs grown as developing sclerotia
	G781P554RA2.T0	340082	340212	249	81.68	52.61	n/a	Ss ESTs grown as developing sclerotia
blastx_uniprot_sprot	O62742	340079	341299	547	56.41	71.30	n/a	Non-specific lipid-transfer protein - Oryctolagus cuniculus (Rabbit)
	P22307	340079	341299	547	55.90	71.30	n/a	Non-specific lipid-transfer protein - Homo sapiens (Human)
	Q07598	340079	341296	547	56.19	70.93	n/a	Non-specific lipid-transfer protein - Gallus gallus (Chicken)
	P07857	340079	341299	543	56.04	71.64	n/a	Non-specific lipid-transfer protein - Bos taurus (Bovine)
	P11915	340079	341299	547	54.10	71.30	n/a	Non-specific lipid-transfer protein - Rattus norvegicus (Rat)
	P32020	340079	341299	547	55.13	71.30	n/a	Non-specific lipid-transfer protein - Mus musculus (Mouse)
	O26884	340607	341302	383	39.47	59.53	n/a	Uncharacterized protein MTH_793 - Methanobacterium thermoautotrophicum
	Q58944	340607	341269	392	34.56	55.36	n/a	Uncharacterized protein MJ1549 - Methanococcus jannaschii
blastx_uniprot_trembl	Q873I6	340052	341485	458	79.48	100.00	n/a	Probable sterol carrier protein - Neurospora crassa
	A1CGU9	340061	341485	458	79.56	99.34	n/a	Sterol carrier protein putative - Aspergillus clavatus
	Q0CL49	340061	341485	458	79.34	99.34	n/a	Nonspecific lipid-transfer protein mitochondrial - Aspergillus terreus (strain NIH 2624)
	Q5B527	340061	341485	458	79.12	99.34	n/a	Putative uncharacterized protein - Emericella nidulans (Aspergillus nidulans)
	Q2UG74	340061	341485	458	78.90	99.34	n/a	Peroxisomal 3-ketoacyl-CoA-thiolase P-44/SCP2 - Aspergillus oryzae
	Q4WNN6	340061	341485	458	78.68	99.34	n/a	Sterol carrier protein putative - Aspergillus fumigatus (Sartorya fumigata)
	Q1DZT6	340073	341485	458	79.16	98.47	n/a	Putative uncharacterized protein - Coccidioides immitis
	A1CXY1	340061	341485	458	78.24	99.34	n/a	Sterol carrier protein putative - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
	Q873I6	340052	341485	458	79.48	100.00	n/a	Probable sterol carrier protein - Neurospora crassa
blastx_FNG30_prot	SS1G_10890.1	340052	341485	433	95.84	100.00	n/a	sclerotinia_sclerotiorum hypothetical protein translation 434 aa
	NCU03372.2	340052	341485	458	79.48	100.00	n/a	Neurospora crassa hypothetical protein translation 459 aa
	AN4353	340061	341485	458	79.12	99.34	n/a	Aspergillus nidulans AN4353.3 conserved hypothetical protein translation 459 aa
	AO090023000951	340061	341485	458	78.90	99.34	n/a	Aspergillus oryzae peroxisomal 3-ketoacyl-CoA-thiolase P-44/SCP2
	gi:70994818:ref:XP_752186.1:	340061	341485	458	78.68	99.34	n/a	Aspergillus fumigatus Af293 sterol carrier protein
	CIMG_04177.2	340073	341485	458	79.16	98.47	n/a	Coccidioides immitis conserved hypothetical protein translation 459 aa
	CHG08079.1	340052	341452	461	78.52	96.96	n/a	Chaetomium globosum conserved hypothetical protein translation 462 aa
	FG10853.1	340052	341485	458	75.11	100.00	n/a	fusarium_graminearum hypothetical protein 459 aa
	SNU05110.1	340055	341485	457	74.62	100.00	n/a	Stagonospora nodorum hypothetical protein translation
	MGG_02409.5	340061	341485	463	73.86	99.14	n/a	magnaporthe_grisea conserved hypothetical protein translation 464 aa
	SS1G_10890.1	340052	341485	433	95.84	100.00	n/a	sclerotinia_sclerotiorum hypothetical protein translation 434 aa
	NCU03372.2	340052	341485	458	79.48	100.00	n/a	Neurospora crassa hypothetical protein translation 459 aa
	CHG08079.1	340052	341452	461	78.52	96.96	n/a	Chaetomium globosum conserved hypothetical protein translation 462 aa
	FG10853.1	340052	341485	458	75.11	100.00	n/a	fusarium_graminearum hypothetical protein 459 aa
sim4_B0510_genes	BC1G_03902.1	340052	341488	1377	99.64	100.00	n/a	n/a
sim4_SS_genes	SS1G_10890.1	340076	341484	1302	85.71	94.09	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.14	337466	347140	433463	99.12	n/a	n/a	n/a
blastn_SS_supercontig	SS_supercontig_1.16	340595	341478	1119265	85.63	n/a	n/a	n/a
	SS_supercontig_1.16	340051	340527	1119265	85.53	n/a	n/a	n/a
	SS_supercontig_1.16	340051	340527	1119265	85.53	n/a	n/a	n/a
blat_oligo	botrytisP00043202	340052	340111	60	n/a	n/a	n/a	array_gene:BofuT4_P008630_1 . max_over_threshold:34.74 . min_over_threshold:0.58 . percent_hyb_over_threshold:92.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00043203	340196	340255	60	n/a	n/a	n/a	array_gene:BofuT4_P008630_1 . max_over_threshold:134.24 . min_over_threshold:2.42 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00043204	340398	340457	60	n/a	n/a	n/a	array_gene:BofuT4_P008630_1 . max_over_threshold:93.66 . min_over_threshold:2.77 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00043205	340422	340481	60	n/a	n/a	n/a	array_gene:BofuT4_P008630_1 . max_over_threshold:120.32 . min_over_threshold:1.83 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00043206	340446	340505	60	n/a	n/a	n/a	array_gene:BofuT4_P008630_1 . max_over_threshold:97.78 . min_over_threshold:2.34 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00043207	340454	340513	60	n/a	n/a	n/a	array_gene:BofuT4_P008630_1 . max_over_threshold:96.53 . min_over_threshold:1.96 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00043208	340680	340739	60	n/a	n/a	n/a	array_gene:BofuT4_P008630_1 . max_over_threshold:79.28 . min_over_threshold:1.94 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00043209	341137	341196	60	n/a	n/a	n/a	array_gene:BofuT4_P008630_1 . max_over_threshold:101.92 . min_over_threshold:1.79 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00043211	341392	341451	60	n/a	n/a	n/a	array_gene:BofuT4_P008630_1 . max_over_threshold:95.61 . min_over_threshold:2.44 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00043202	340052	340111	60	n/a	n/a	n/a	array_gene:BofuT4_P008630_1 . max_over_threshold:34.74 . min_over_threshold:0.58 . percent_hyb_over_threshold:92.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog