Study : The Chloroplast RNA Binding Protein CP31A Has a Preference for mRNAs Encoding the Subunits of the Chloroplast NDH Complex and is Required for Their Accumulation

Identification

• Name: The Chloroplast RNA Binding Protein CP31A Has a Preference for mRNAs Encoding the Subunits of the Chloroplast NDH Complex and is Required for Their Accumulation
• Identifier: dXJuOkVWQS9zdHVkeS9QUkpOQTYzOTc4Mg==
• Data link: Link to this study on EBI European Nucleotide Archive
• Description: Chloroplast RNA processing requires a large number of nuclear-encoded RNA binding proteins (RBPs) that are imported post-translationally into the organelle. Most of these RBPs are highly specific for one or few target RNAs. By contrast, members of the chloroplast ribonucleoprotein family (cpRNPs) have a wider RNA target range. We here present a quantitative analysis of RNA targets of the cpRNP CP31A using digestion-optimized RNA co-immunoprecipitation with deep sequencing (DO-RIP-seq). This identifies the mRNAs coding for subunits of the chloroplast NDH complex as main targets for CP31A. We demonstrate using whole-genome gene expression analysis and targeted RNA gel blot hybridization that the ndh mRNAs are all down-regulated in cp31a mutants. This diminishes the activity of the NDH complex. Our findings demonstrate how a chloroplast RNA binding protein can combine functionally related RNAs into one post-transcriptional operon. Overall design: RIP-seq of CP31A in Arabidopsis thaliana WT plants with 3 replicates
• Data files: SRR12023965; SRR12023964; SRR12023963; SRR12023962; SRR12023961; SRR12023960