Study : The impact of cold priming on the light and cold regulation of gene expression in Arabidopsis thaliana
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Identification
Name
The impact of cold priming on the light and cold regulation of gene expression in Arabidopsis thaliana
Identifier
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Description
We analysed the effect of cold priming on cold and high light regulation of gene expression. 5 days after the first cold treatment the primary stress response was widely reset. Then, a second (triggering) cold stimulus (24 h 4 °C) and a triggering high-light stimulus (2 h 800 µmol quanta m-2 s-1), which regulate many stress responsive genes in the same direction in naïve plants, caused widely specific and even inverse regulation of priming-responsive genes. Overall design: Transcript levels were assessed in a transcriptome wide scale by single end next generation sequencing (Illumina High-Seq4000). Half of the 4-week old Arabidopsis thaliana Col-0 plants were primed for 24 hours at 4 °C. 5 days after priming, 1/3 of the non-primed and 1/3 of the cold-primed plants were harvested before triggering (non-primed control plants = C, primed plants = P). Half of the remaining primed and half of the remaining cold-primed plants were cold triggering for 24 hours at 4 °C (cold primed + cold triggered = PT-C; only cold trigger = T-C). The other plants were exposed for two hours at 800 μmol quanta*m−2 s−1 (cold primed + light triggered = PT-L ; only light trigger = T-L), which similarly induces the cold-primable gene ZAT10 in naïve plants. Triggered plants were harvested 2 h after the end of the triggering treatment.
Genotype
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