Study : Arabidopsis NRP switches off the PP6 phosphatase FyPP3 to repress auxin-mediated growth in response to abscisic acid

Identification

Name

Arabidopsis NRP switches off the PP6 phosphatase FyPP3 to repress auxin-mediated growth in response to abscisic acid

Identifier

dXJuOkVWQS9zdHVkeS9QUkpOQTYzMzA5Ng==

Source

Data link

Link to this study on EBI European Nucleotide Archive

Description

How environmentally challenged plants can switch from growth to stress adaptation is an important question in plant physiology. To identify the key regulatory proteins and to characterize the underlying signaling pathways are essential in answering this question. Here we show that the abiotic stress hormone Abscisic acid (ABA) induces the expression of the Asparagine rich proteins (NRP), DCD1 and DCD2, to repress the auxin-induced growth by promoting vacuolar degradation of PIN2, the auxin efflux carrier. ABA-induced degradation of PIN2 is inhibited in the dcd1 dcd2 double mutant, and inhibition of FyPP3, the catalytic subunit of the PP6 protein phosphatase, can restore the PIN recycling and growth phenotype in the double mutant. Transcriptome analyses on WT, dcd1 dcd2, the FyPP3 Dominant Negative line (FyPP3-DN), and dcd1 dcd2/FyPP3-DN supported the essential function of the NRP-FyPP3 module in the growth- to stress-response switch. Overall design: Arabidoposis thaliana mRNA extracted from 7-day old wild type (WT), nrp1 nrp2, FyPP3DN and nrp1 nrp2 / FyPP3DN seedling (6 days for growing and 1day treated with 5 μM ABA or not), deep sequencing, in triplicate, using Illumina HiSeq 2500 system

Data files

Genotype

Accession number	Name	Taxon