Study : Identification of SHOT1/MTERF18 binding sites on the mitochondrial genome of Arabidopsis thaliana

Identification

• Name: Identification of SHOT1/MTERF18 binding sites on the mitochondrial genome of Arabidopsis thaliana
• Identifier: dXJuOkVWQS9zdHVkeS9QUkpOQTYzMTY5MQ==
• Data link: Link to this study on EBI European Nucleotide Archive
• Description: To identify possible mitochondrial DNA binding targets of SHOT1/MTERF18 (AT3G60400), C-terminal GFP fusion constructs under either a constitutive 35S promoter (35S::SHOT1-GFP) or the SHOT1 native promoter (SHOT1p::SHOT1-GFP) were made. The transgenes were introduced into the shot1-2 mutant background (Kim et al., 2012, Plant Cell) and verified to be functional based on recovery of shot1 from growth defects. As a control, a transgenic line harboring mitochondria-targeted GFP (Mito-GFP) under the 35S promoter was used. All enriched peaks in SHOT1-GFP samples are located upstream of different tRNA genes: trnS, trnM, trnG and trnF Overall design: Immunoprecipitation of SHOT1-GFP (using GFP-Trap (ChromoTek)) crosslinked to DNA from isolated mitochondria of Mito-GFP (control), 35S::SHOT1-GFP (MK30) and SHOT1p::SHOT1-GFP (MK108) plants
• Data files: SRR11768234; SRR11768233; SRR11768235