Study : URT1-mediated uridylation shapes poly(A) tails in Arabidopsis

Identification

• Name: URT1-mediated uridylation shapes poly(A) tails in Arabidopsis
• Identifier: dXJuOkVWQS9zdHVkeS9QUkpOQTYyNDE0NQ==
• Data link: Link to this study on EBI European Nucleotide Archive
• Description: Using 3 RACE-seq method, we show that the Arabidopsis TUTase URT1 shapes poly(A) tail profiles by reducing the accumulation of short-tailed mRNAs in planta. We took advantage of the depth of this method to precisely compare polyadenylation and uridylation profiles for 22 mRNAs analyzed in two biological replicates of wild-type and urt1 plants in Arabidopsis Overall design: 3’ RACE-seq method was used to measure poly(A) tail sizes and analyze uridylation levels. This protocol is based on the ligation of a primer at the 3’ end of RNA, and the subsequent targeted amplification by PCR of amplicons suitable for Illumina sequencing.
• Data files: SRR11521300; SRR11521299; SRR11521298; SRR11521297; SRR11521296; SRR11521295; SRR11521294; SRR11521293; SRR11521292; SRR11521291; SRR11521290; SRR11521289; SRR11521288; SRR11521287; SRR11521286; SRR11521285; SRR11521284; SRR11521283; SRR11521282; SRR11521281