Study : Transcriptomic analysis reveals Arabidopsis Auxin Resistant 1 gene as a key mediator for responses to bacteria.

Identification

Name

Transcriptomic analysis reveals Arabidopsis Auxin Resistant 1 gene as a key mediator for responses to bacteria.

Identifier

dXJuOkVWQS9zdHVkeS9QUkpOQTYxMDQxNw==

Source

Data link

Link to this study on EBI European Nucleotide Archive

Description

The role of auxin in plant-microbe interaction has been primarily studied using indole-3-acetic acid (IAA)-producing pathogenic or plant growth promoting bacteria. However, the IAA biosynthesis pathway in bacteria involves indole related compounds and intermediates with lesser known functions. Here, we seek to understand changes in plant response to various bacteria strains that differ in their ability to produce indole related compounds. We previously isolated 47 bacterial strains from various duckweed ecotypes and determined 79% produced indole related compounds in culture, such as IAA, indole-lactic acid (ILA) and indole. Using Arabidopsis thaliana, we performed binary-association assays on a subset of these strains to evaluate physiological and transcriptomic responses in the plant. High quantity IAA-producing Microbacterium strains caused a short root phenotype on Arabidopsis, while a lower quantity IAA-producing Microbacterium strain affected auxin response genes in the absence of an obvious root phenotype. Use of the auxin insensitive mutants axr1-3 and tmk1 tmk3 TMK4/tmk4 revealed evidence for diverse functions of the AXR1 gene that may be related to bacterial interaction in addition to the IAA response. Moreover, our findings provided additional insights to the complex roles of IAA in plant-microbe interactions. Our results highlight the key role that AXR1 may play during the process of plant-bacteria interaction in general.

Data files

Genotype

Accession number	Name	Taxon