Study : RNA-seq analysis of WT and bdt1 in Arabidopsis.
RNA-seq analysis of WT and bdt1 in Arabidopsis.
Trimethylated histone H3 lysine 27 (H3K27me3) is a repressive histone marker that regulates a variety of developmental processes, including those that determine flowering time. However, relatively little is known about the mechanism of how H3K27me3 is recognized to regulate transcription. We identified BAH domain-containing transcriptional regulator 1 (BDT1) as an H3K27me3 reader. BDT1 is responsible for preventing flowering by suppressing the expression of flowering genes. Mutation of the H3K27me3 recognition sites in the BAH domain disrupted the binding of BDT1 to H3K27me3, leading to de-repression of H3K27me3-enriched flowering genes and an early-flowering phenotype. Overall design: We perfomed RNA seq on wild type and bdt1 grown for 10 days in long-day conditions. Two biological replicates per genotype were sequenced.