Study : Manihot esculenta Transcriptome (TAL Effector Repertoires of Strains of Xanthomonas phaseoli pv. manihotis in Commercial Cassava Crops Reveal High Diversity at the Country Scale)
Identification
Name
Manihot esculenta Transcriptome (TAL Effector Repertoires of Strains of Xanthomonas phaseoli pv. manihotis in Commercial Cassava Crops Reveal High Diversity at the Country Scale)
Identifier
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Source
Description
Transcription activator-like effectors (TALEs) play a significant role for pathogenesis in several xanthomonad pathosystems. Xanthomonas phaseoli pv. manihotis (Xpm), the causal agent of Cassava Bacterial Blight (CBB), uses TALEs to manipulate host metabolism. Information about Xpm TALEs and their target genes in cassava is scarce, but has been growing in the last few years. We aimed to characterize the TALE diversity in Colombian strains of Xpm and to screen for TALE-targeted gene candidates. We selected eighteen Xpm strains based on neutral genetic diversity at a country scale to depict the TALE diversity among isolates from cassava productive regions. RFLP analysis showed that Xpm strains carry TALomes with a bimodal size distribution, and affinity-based clustering of the sequenced TALEs condensed this variability mainly into five clusters. We report on the identification of 13 novel variants of TALEs in Xpm, as well as a functional variant with 22 repeats that activates the susceptibility gene MeSWEET10a, a previously reported target of TAL20Xam668. Transcriptomics and EBE prediction analyses resulted in the selection of several TALE-targeted candidate genes and two potential cases of functional convergence. This study provides new bases for assessing novel potential TALE targets in the Xpm-cassava interaction, which could be important factors that define the fate of the infection.In this study, two strains of Xanthomonas phaseoli pv. manihotis (UA681 and UA1061) and a mock treatment (sterile 10-mM MgCl2 solution) were inoculated in leaves of 1-month-old cassava in-vitro plants from the variety 60444. Inocula were adjusted to an OD600 of 0.02 (ca. 2 x 107 cfu/mL) in a 10-mM MgCl2 solution. In-vitro propagated plants were inoculated with bacterial suspensions or mock solution (10-mM MgCl2) with a swab on axial and abaxial surfaces of punctured leaves (nine needle punctures per leaf); each treatment was inoculated on three leaves per plant and on three different plants. Tissue surrounding inoculated punctures was collected at 50 hpi using a 3-mm diameter cork borer.
Genotype
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