Study : Vitis vinifera Raw sequence reads CPPU and GA experiment
Identification
Name
Vitis vinifera Raw sequence reads CPPU and GA experiment
Identifier
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Source
Description
Experiment was carried out on Vitis vinifera cv. Sugrasixteen (SABLE SEEDLESS) (Sun World, Bakersfield, CA) that will be referred as Sable. Vines of Sable were grafted on Richter rootstock that was 7 years old and grown in Israel in the Lachish area . All the viticulture practice were performed as describe previously23. The experimental plot comprised 4 replications of 3 vines each arranged in randomized block design. Vine were treated with growth regulators gibberellic acid (GA3, ProGibb 40, Valent BioScience, Walnut Creek, CA, USA), forchlorfenuron (CPPU, a synthetic cytokinin, SKW, Wittenberg, Germany) in a concentration of 20 and 5 ppm, respectively. A combination of GA and CPPU (hereafter GA+CPPU) were also sprayed on the berry with same concentration. Sable was treated on 14th of May 2018 at the berry diameter of 6.0 mm. Phenological data for Sable was collected at the time of treatment, 7, 34, 51 and at 70 d. Sampling for metabolites, volatiles and gene expression was carried out at 51 and 70 d after treatment, time points which represent the beginning and end of commercial harvest. Sampling at all-time points was of 90 berries from 20 to 30 clusters randomly collected from the 4 replications. Discs of 14-16 mm were cut along the longitudinal axis of the berry and frozen in liquid nitrogen. The frozen samples (30 discs) were homogenized in three replicates using an IKA homogenizer (IKA, Staufen, Germany) with liquid nitrogen and stored at -80 C for further analysis.
Genotype
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