Study : maize DDM1 targets RNA-directed DNA methylation on active chromatin

Identification

• Name: maize DDM1 targets RNA-directed DNA methylation on active chromatin
• Identifier: dXJuOkVWQS9zdHVkeS9QUkpOQTY4MjYwNw==
• Data link: Link to this study on EBI European Nucleotide Archive
• Description: In this work, we used chromatin immunoprecipitation coupled with high throughput sequencing (ChIP-seq) to ascertain the genome-wide occupancy of ZmDDM1 in maize genome. The results revealed that ZmDDM1 recognized an 8-bp-long GC-rich degenerate DNA sequence motif, which is enriched in transcription start sites (TSSs) and other euchromatic regions. Meanwhile, 24-nucleotide siRNAs and CHH methylation were delineated at the edge of ZmDDM1-occupied sites. ZmDDM1 could be co-purified with ZmAGO4 proteins, providing further evidence that ZmDDM1 is a component of RdDM complex in planta. Overall design: ZmDDM1 and ZmAGO4 ChIP-seq in maize developing embryo
• Data files: SRR13198941; SRR13198940; SRR13198939; SRR13198936; SRR13198935; SRR13198934; SRR13198933; SRR13198932; SRR13198938; SRR13198937; SRR13198959; SRR13198957; SRR13198956; SRR13198955; SRR13198954; SRR13198953; SRR13198952; SRR13198958; SRR13198951; SRR13198950