Study : Transcriptional responses of wild-type and zin1 mutant Arabidopsis plants to infection with Pseudomonas syringae
Identification
Name
Transcriptional responses of wild-type and zin1 mutant Arabidopsis plants to infection with Pseudomonas syringae
Identifier
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Description
Phytopathogens use secreted effector proteins to suppress host immunity and promote pathogen virulence, and there is increasing evidence that the host-pathogen interactome comprises a complex network. In an effort to identify novel interactors of the Pseudomonas syringae effector HopZ1a, we performed a yeast two-hybrid screen that identified a previously uncharacterized Arabidopsis protein that we designate HopZ1a Interactor 1 (ZIN1). Additional analyses in yeast and in planta revealed that ZIN1 also interacts with several other P. syringae effectors. We show that an Arabidopsis loss-of-function zin1 mutant is less susceptible to infection by certain strains of P. syringae, while overexpression of ZIN1 results in enhanced susceptibility. Functionally, ZIN1 exhibits topoisomerase-like activity in vitro. Transcriptional profiling of wild-type and zin1 Arabidopsis plants inoculated with P. syringae indicated that ZIN1 regulates a wide range of pathogen-responsive biological processes, although the list of genes more highly expressed in zin1 versus wild-type plants was particularly enriched for ribosomal protein genes. Altogether, these data illuminate ZIN1 as a potential susceptibility hub that interacts with multiple effectors to influence the outcome of plant-microbe interactions. Overall design: Wild-type and zin1 mutants infiltrated with either 10 mM MgCl2 (Control), 1 x 10^8 cfu/mL Pseudmonas syringae pv. tomato DC3000, or 1 x 10^8 cfu/mL P. syringae pv. tomato DC3000 ΔhrcC (three biological replicates each for a total of 18 samples)
Genotype
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