Study : Single cell level analysis of the Arabidopsis root transcriptome and chromatin accessibility
Identification
Name
Single cell level analysis of the Arabidopsis root transcriptome and chromatin accessibility
Identifier
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Description
The nuclei of Arabidopsis root seedlings were isolated then use to establish a single cell level resolution transcriptome (sNucRNA-seq) and profile of chromatin accessibility (sNucATAC-seq) applying 10X Genomics technologies. Overall design: The nuclei of the root of 7 days-old Arabidopsis seedlings grown on agar MS medium were isolated. Upon isolation, the nuclei suspension was used to create sNucRNA-seq and sNucATAC-seq libraries. Five and two independent replicates were generated to perform these experiments., respectviely. The libraries were sequenced using high-throughput sequencing technologies. The data were analyzed using CellRanger, Seurat and Signac.
Genotype
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