Study : Transcriptomic changes in Arabidopsis thaliana upon the expression of mutated TFIIS

Identification

Name
Transcriptomic changes in Arabidopsis thaliana upon the expression of mutated TFIIS
Identifier
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Source
Description
The expresion of dominant negative version of Arabidopsis TFIIS (TFIISmut) in plants lacking funtional TFIIS leads to severe growth defects. The genome-wide analysis of transcriptomic changes upon TFIISmut expression was performed by RNA-seq to better understand the biological importance of TFIIS. 10DAS seedlings of transgenic lines carring inducible GFP-TFIIS or GFP-TFIISmut transgenes were studied by RNA-seq following plants exposure to 24h b-estradiol or EtOH (mock) induction. Overall design: Arabidopsis mRNA profiles of 10-day old transgenic plants with inducible expression of GFP-TFIIS or GFP-TFIISmut fusion proteins in tfIIs-1 background were generated by deep sequencing and analysed comparatively with uninduced plants.

Genotype

Accession number Name Taxon