Study : Genome-wide changes in active RNAPII occupancy in the presence of mutated TFIIS (TFIISmut)
Identification
Name
Genome-wide changes in active RNAPII occupancy in the presence of mutated TFIIS (TFIISmut)
Identifier
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Source
Description
The expresion of dominant negative version of Arabidopsis TFIIS (TFIISmut) in plants lacking funtional TFIIS leads to severe growth defects. The genome-wide analysis of changes in active RNAPII occupancy upon TFIISmut expression was determined using ChIP-seq in order to better understand the biological importance of TFIIS. 10DAS seedlings of transgenic lines carring inducible GFP-TFIIS or GFP-TFIISmut transgenes were studied by ChIP-seq following plants exposure to 24h b-estradiol or EtOH (mock) induction. Overall design: Chromatin DNA immunoprecipitation was performed to determine active RNAPII occupancy using antbodies directed agains S2P-RNAPII or S5P-RNAPII. RNAPII occupancy was studied in 10-day old transgenic plants with inducible expression of GFP-TFIIS or GFP-TFIISmut fusion proteins and analysed comparatively with uninduced plants.
Genotype
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