Study : Identification and Temporal Expression Analysis of Conserved and Novel MicroRNAs in the Leaves of Winter Wheat Grown in the Field
Identification and Temporal Expression Analysis of Conserved and Novel MicroRNAs in the Leaves of Winter Wheat Grown in the Field
MicroRNAs (miRNAs) are small non-coding RNAs that regulate target mRNAs by inducing degradation or preventing translation of their target mRNAs. Winter wheat, Triticum aestivum., is an important crop plant, yet there are only a few studies on the association of miRNAs and growth and development of winter wheat grown in the field. Here we carried out experimental analysis of miRNAs in wheat leaves by analyzing small RNA profiles at different growth stages. Overall design: To identify miRNAs involved in winter wheat growth and development, we performed high-throughput sequencing of sRNA libraries from leaves at 4 different growth and development stages: three leaf stage, winter dormancy stage, spring greenup stage and jointing stage.Winter wheat cultivar Shimai 22 was grown in the field under natural weather condition;BGISEQ500 RS (BGI, China)platform was used.