Study : Landscape of RNA NAD+ capping in Arabidopsis seedling with ABA treatment

Identification

• Name: Landscape of RNA NAD+ capping in Arabidopsis seedling with ABA treatment
• Identifier: dXJuOkVWQS9zdHVkeS9QUkpOQTU5NjgwMw==
• Data link: Link to this study on EBI European Nucleotide Archive
• Description: Eukaryotic messenger RNAs (mRNAs) possess a 5’-end N7-methyl guanosine (m7G) cap that promotes their translation and stability. However, it was recently demonstrated that eukaryotic mRNAs can also carry a 5 end nicotinamide adenine dinucleotide (NAD+) cap that promotes mRNA decay mediated by the NAD+ decapping enzyme DXO1. However, the dynamic regulation of NAD+ capping in plant remains unknown. Here, we describe the global landscape of NAD+-capped RNAs in Arabidopsis thaliana, and demonstrate that DXO1 is responsible for removal of these 5’-end modifications and facilitates mRNA degradation in plant transcriptomes. We also reveal that in the absence of DXO1 NAD+-capped mRNAs are unstable and processed into smRNAs. Furthermore, we find that Abscisic Acid (ABA) remodel the landscape of RNA cap epitransciptome, and the mRNA lost their NAD+ cap contribute to their stability under ABA. Overall, our results support a link between ABA response and RNA NAD+ capping. Overall design: NADseq, GMUCT and total RNAseq in Arabidopsis transcriptome with ABA treatment
• Data files: SRR10742186; SRR10742185; SRR10742184; SRR10742183; SRR10742182; SRR10742181; SRR10742180; SRR10742179; SRR10742178; SRR10742177; SRR10742176; SRR10742175; SRR10742174; SRR10742173; SRR10742172; SRR10742171; SRR10742170; SRR10742169; SRR10742168; SRR10742167; ERZ1735968; ERZ1735965; ERZ1735960; ERZ1735974; ERZ1735966; ERZ1735988; ERZ1735970; ERZ1735994; ERZ1735987; ERZ1735979; ERZ1735981; ERZ1735984; ERZ1735983; ERZ1735972; ERZ1735990; ERZ1735991; ERZ1735976