Study : ZmNF-YB16-YC17-YA1 complex participates in the regulation of plant growth and drought resistance in maize

Identification

• Name: ZmNF-YB16-YC17-YA1 complex participates in the regulation of plant growth and drought resistance in maize
• Identifier: dXJuOkVWQS9zdHVkeS9QUkpOQTU3MzA1NA==
• Data link: Link to this study on EBI European Nucleotide Archive
• Description: The identification of drought stress regulatory genes is crucial for the genetic improvement of maize yield. NF-Ys are important transcription factors, but roles in the drought stress resistance of plants and underlying molecular mechanism are largely unknown. In this work, we identified two heterotrimer complexes in maize, ZmNF-YA1-YB16-YC17 and ZmNF-YA7-YB16-YC17. ZmNF-YB16 interacts with ZmNF-YC17 via its histone fold domain to form a heterodimer in the cytoplasm, and then, the latter enters the nucleus to form a heterotrimer with ZmNF-YA1 or ZmNF-YA7 when subjected to osmotic stress. Overexpression of ZmNF-YA1 improves the abiotic stress tolerance and root development of maize, while zmnf-ya1 mutants exhibit abiotic stress sensitivity. A transactivation assay in yeast demonstrates that ZmNF-YA1 does not show transcription activation activity, in contrast to ZmNF-YB17 and ZmNF-YC17. ZmNF-YA1 can bind to the promoter CCAAT motif, and the ZmNF-YA1-YB17-YC17 trimer can directly regulate the expression of multiple genes, such as ZmbHLH116, ZmPOD64, ZmLOX5, and ZmCNR9. These target genes play important roles in the stress response and plant development. The transcriptome comparison and characteristic analysis between ZmNF-YB16 OE and ZmNF-YA1 OE lines show that ZmNF-YA1 and ZmNF-YB16 have similar biological functions in the stress response process, but diacritical functions in other biological processes. Taken together, ZmNF-YA1 is a positive regulator of the plant abiotic stress response and involved in the root development of maize, and ZmNF-YA-YB-YC complexes with different subunits may have discrepant functions. Overall design: Examination of 6 different materials (B104, ZmNF-YB16 OE, DH4866, ZmNF-YA1 OE, W22, zmnf-ya1 mutant ) under normal and drought conditions
• Data files: SRR10153115; SRR10153114; SRR10153113; SRR10153112; SRR10153111; SRR10153110; SRR10153109; SRR10153108; SRR10153107; SRR10153106; SRR10153105; SRR10153104; SRR10153103; SRR10153102; SRR10153101; SRR10153100; SRR10153123; SRR10153122; SRR10153121; SRR10153120; ERZ1720065; ERZ1720075; ERZ1720067; ERZ1720076; ERZ1720052; ERZ1720081; ERZ1720066; ERZ1720071; ERZ1720074; ERZ1720064; ERZ1720063; ERZ1720072; ERZ1720042; ERZ1720073; ERZ1720057; ERZ1720069; ERZ1720078; ERZ1720068; ERZ1720077; ERZ1720070