Study : Circadian clock regulation of alternative splicing and alternative polyadenylation in Arabidopsis thaliana
Identification
Name
Circadian clock regulation of alternative splicing and alternative polyadenylation in Arabidopsis thaliana
Identifier
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Source
Description
Purpose: Circadian clock in plants temporally coordinates biological processes throughout the day synchronizing gene expression with environmental changes. Here, we examined the genome-wide circadian and diurnal control of Arabidopsis transcriptome using high throughout RNA-seq approach. Methods: Transcriptional and posttranscritional profiles were identified and characterized for Arabidopsis seedlings grown under continuous light or long-day condition (16 h light/8 h dark) for one day (each condition has two biological replicates). Results: We show that rhythmic posttranscriptional regulation is also a significant factor for genome-wide profile of circadian plant transcriptome. Two major posttranscriptioal mechanisms alternative splicing (AS) and alternative polyadenylation (APA) show circadian rhythmicity, resulting from the oscillation in the genes invovled in AS and APA. Conclusions: Arabidopsis circadian clock not only controls the transcription of genes, but also affects their posttranscriptional regulation through regulating AS and APA. Overall design: Posttranscriptional profiles of Arabidopsis seedlings grown under continuous light or long-day condition (16 h light/8 h dark) for one day were generated using Illumina HiSeq 4000.
Genotype
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