Study : Time course of the Arabidopsis root stem cells

Identification

Name
Time course of the Arabidopsis root stem cells
Identifier
dXJuOkVWQS9zdHVkeS9QUkpOQTU0NTUxNQ==
Source
EBI European Nucleotide Archive logo
Data link
Link to this study on EBI European Nucleotide Archive
Description
The goal of this dataset was to transcriptionally profile the Arabidopsis root stem cells over developmental time. To accomplish this, we protoplasted roots expressing PET111:GFP every 8 hours from 4 days to 6 days old. We then collected GFP negative cells from the PET111:GFP line using cell sorting, as these cells represent the stem cell population. RNA was extracted from cells, libraries were prepared, and RNA-seq was performed. FPKM values were calulcated using Cufflinks. Overall design: Arabidopsis roots expressing PET111:GFP were protoplasted every 8 hours from 4 days to 6 days post-stratification. GFP-negative cells were collected, RNA was extracted, and RNA-seq was performed.
Data files

Genotype

Accession number Name Taxon