Study : Genome-wide occupancy of BDR1 and BDR3 (ChIP-seq)

Identification

• Name: Genome-wide occupancy of BDR1 and BDR3 (ChIP-seq)
• Identifier: dXJuOkVWQS9zdHVkeS9QUkpOQTU0NDc5OQ==
• Data link: Link to this study on EBI European Nucleotide Archive
• Description: We generated Arabidopsis lines expressing tagged (3X Myc tag) versions of BDR1 or BDR3 driven by their endogenous promoters on a bdrs triple mutant background (bdrs: cross of bdr1/AT5G25520 mutant SALK_142108C, bdr2/AT5G11430 mutant CS852350 and bdr3/AT2G25640 mutant SALK_059905). The genome-wide distribution of BDR1 and BDR3 in 8-day old seedlings were analyzed by ChIP-seq using an anti Myc antibody. As control, we performed in parallel a ChIP in wild-type seedlings using the anti-Myc tag antibody. We found that BDR1 is enriched at the border of a large number of genes in Arabidopsis genome while BDR3 is mostly enriched at the 3-end of genes. Overall design: ChIP-seq profiles in 8-day old seedlings of Myc tag-BDR1 and Myc tag-BDR3 (Myc tag-BDR proteins expressed in bdrs triple mutant background). Control is a ChIP perfomed in wild-type plants (not expressing any tagged protein) with the anti-Myc tag antibody.
• Data files: SRR9126613; SRR9126612; SRR9126611; SRR9126610