Study : Transcriptomics of the plant growth regulator Heatin

Identification

• Name: Transcriptomics of the plant growth regulator Heatin
• Identifier: dXJuOkVWQS9zdHVkeS9QUkpOQTU0MjExNQ==
• Description: Temperature passively affects many biological processes. It is therefore challenging to study dedicated temperature signalling pathways orchestrating plant thermomorphogenesis; a suite of elongation growth-based adaptations that enhance leaf cooling capacity. Following a chemical genetics approach, we screened a chemical library for compounds that restored abolished hypocotyl elongation in the pif4-2 deficient mutant background in the model plant Arabidopsis thaliana. The small aromatic compound ‘Heatin (N-[(2-hydroxy-1-naphthyl)methylene]-2-(1-naphthylamino)propanohydrazide), with 1-aminomethyl-2-naphthol, as minimal active moiety, was isolated as potent enhancer of elongation growth. Here we assessed the transcriptomic changes induced by the compound, by supplementing the MS-agar growth medium with Heatin compared to DMSO solvent, in whole seedlings of the Col-0 wild type background in control (22oC) and high ambient temperature conditions (27oC). Overall design: Pooled seedlings, grown on sterile MS-agar plates, harvested at dawn of day 3 after initiation of germination (2 day old) and of day 8 (7 day old), in 3 replicates per mutant of 3 pooled independent batches (>50 seedlings per batch), genotype and temperature environment in short day conditions.
• Active: No
• Data files: SRR9034707; SRR9034706; SRR9034705; SRR9034704; SRR9034703; SRR9034702; SRR9034701; SRR9034700; SRR9034699; SRR9034698; SRR9034697; SRR9034708; SRR9034715; SRR9034714; SRR9034713; SRR9034720; SRR9034719; SRR9034718; SRR9034717; SRR9034716; ERZ1290233; ERZ1290239; ERZ1290228; ERZ1290220; ERZ1290229; ERZ1290234; ERZ1290227; ERZ1290231; ERZ1290219; ERZ1290236; ERZ1290223; ERZ1290222; ERZ1290235; ERZ1290225; ERZ1290232; ERZ1290226; ERZ1290224; ERZ1290230