Study : Glycerol biosynthesis pathways from starch endowing Dunaliella salina adaptations to osmotic and oxidative stresses caused by salinity
Identification
Name
Glycerol biosynthesis pathways from starch endowing Dunaliella salina adaptations to osmotic and oxidative stresses caused by salinity
Identifier
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Source
Description
Dunaliella salina, a unicellular and eukaryotic alga, has been found as one of the most salt-tolerant eukaryote with a short growth period and wide practical applications. To elucidate the underlying molecular mechanism involved in the response to salinity and its different effects, RNA-seq was used for global transcriptome profiling of D. salina exposed to NaCl, Sorbiol and H2O2 stress. To maintain osmotic pressure homeostasis under suboptimal environment condition, starch breakdown catalyzed by both alpha-amylase (AMY) and glycogen phosphorylase (PYG) with consecutive expression patterns and low activity of PYG at the beginning of salt stress might be caused by a shortage of ATP because of impaired photosynthesis. Moreover, clustering analysis of differentially expressed genes (DEGs) indicated that starch and sucrose metabolism as well as glycerol metabolism reprogrammed under high salt stress only. For redox homeostasis, glycerol-3-phosphate shuttle performed by mitochondrial glycerol-3-phosphate dehydrogenases (GPDHs) participates the redox imbalance under abiotic stresses. c23777_g1 is a gene of D. salina involved in glycerol 3 phosphate (G3P) shuttle under various abiotic stresses while c25199_g1 is a gene of G3P shuttle induced only by osmotic stress. Overall design: Total mRNA profiles of D. salina exposed to NaCl, Sorbiol and H2O2 stress were generated by deep sequencing, in triplicate, using Illumina NextSeq500.
Genotype
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