Study : Identification of LEC1 binding sites in Arabidopsis seeds during embryo expansion stage
Identification of LEC1 binding sites in Arabidopsis seeds during embryo expansion stage
LEAFY COTYLEDON1 (LEC1), a subunit of the NF-Y CCAAT binding transcription factor, is a key regulator that controls many aspects of seed development including embryo growth and seed storage accumulation. To identified genomic region bound by LEC1 and target genes of LEC1, we expressed a FLAG-tagged form of LEC1 under the control of its native promoter and terminator in lec1-4 mutant seeds. Using an anti-FLAG antibody (Sigma), we performed chromatin immunoprecipitation followed by sequencing (ChIP-Seq) using chromatin isolated from mature stage seeds to identify the genes bound by LEC1. These data reveal that the transcription regulation network of LEC1. Overall design: Identification of LEC1 binding sites in arabidopsis seeds at seed mature stages. ChIP and total chromatin controls (input) were were generatedby deep sequencing, with two biological replicates, using Illumina HiSeq 2500. Please note that the peak files were generated from both ChIP and input samples together and are linked to the correspdonding ChIP sample records.