Study : A DNA methylation reader complex that enhances gene transcription

Identification

• Name: A DNA methylation reader complex that enhances gene transcription
• Identifier: dXJuOkVWQS9zdHVkeS9QUkpOQTQyNzIwMg==
• Data link: Link to this study on EBI European Nucleotide Archive
• Description: DNA methylation generally functions as a repressive transcriptional signal, but it is also​ ​known to activate gene expression. In either case, the downstream factors remain​ ​largely unknown. By using comparative interactomics, we isolated proteins in Arabidopsis​ ​thaliana that associate with methylated DNA. Two SU(VAR)3-9 homologs, the transcriptional​ ​antisilencing factor SUVH1, and SUVH3, were among the methyl reader candidates. SUVH1​ ​and SUVH3 bound methylated DNA in vitro, were associated with euchromatic methylation in​ ​vivo, and formed a complex with two DNAJ domain-containing homologs, DNAJ1 and DNAJ2.​ ​Ectopic recruitment of DNAJ1 enhanced gene transcription in plants, yeast, and mammals.​ ​Thus, the SUVH proteins bind to methylated DNA and recruit the DNAJ proteins to enhance​ ​proximal gene expression, thereby counteracting the repressive effects of transposon​ ​insertion near genes. Overall design: This dataset includes: 4 ATAC-seq, 26 ChIP-seq, 14 whole genome bisulfite seq (WGBS) and 45 RNAseq datasets.
• Data files: SRR6410821; SRR6410860; SRR6410859; SRR6410858; SRR6410857; SRR6410856; SRR6410855; SRR6410854; SRR6410853; SRR6410852; SRR6410850; SRR6410849; SRR6410888; SRR6410887; SRR6410886; SRR6410885; SRR6410884; SRR6410883; SRR6410882; SRR6410881; ERZ1179311; ERZ1179306; ERZ1179346; ERZ1179298; ERZ1179321; ERZ1179313; ERZ1179319; ERZ1179302; ERZ1179345; ERZ1179330; ERZ1179325; ERZ1179308; ERZ1179349; ERZ1179290; ERZ1179339; ERZ1179344; ERZ1179300; ERZ1179338; ERZ1179337; ERZ1179317