Study : Formaldehyde-crosslinking and immunoprecipitation (FA-CLIP) sequencing to identify ECT2 targets in Arabidopsis
Identification
Name
Formaldehyde-crosslinking and immunoprecipitation (FA-CLIP) sequencing to identify ECT2 targets in Arabidopsis
Identifier
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Description
Developed an efficient method to identify RNA binding sites for RNA-binding proteins in plants. Overall design: Formaldehyde-crosslinking enhance the binding affinity of RNA-binding protein of RNA, while RNaseT1 digestion can improve the resolution of targets site. We combined the two methods to generate FA-CLIP method in plant to identify RNA-binding protein targets.
Genotype
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