Study : Trifolium cultivar:yanninicum Raw sequence reads
Identification
Name
Trifolium cultivar:yanninicum Raw sequence reads
Identifier
dXJuOkVWQS9zdHVkeS9QUkpOQTQwNDAxMw==
Source
Description
Background: Whole genome sequencing has been widely used to detect structural variations (SVs). However, the limited single molecule size makes it difficult to characterize large-scale SVs in a genome because they cannot fully cover such vast and complex regions. Recently, optical mapping in nanochannels has provided novel resolution to detect large-scale SVs by comparing the physical location of particular enzymes in genomes. Other than in humans, SVs discovered in plants by optical mapping have not been validated.Results: To assess the accuracy of SV calling in plants by optical mapping, we selected two genetically diverse subspecies of the Trifolium model species, subterranean clover cvs. Daliak and Yarloop. The SVs discovered by BioNano optical mapping (BOM) were validated using Illumina short reads. In the analysis, BOM identified 12 large-scale regions containing deletions and 19 containing insertions in Yarloop. The 12 large-scale regions contained 71 small deletions when validated by Illumina short reads. The results suggest that BOM could detect the total size of deletions and insertions, but it could not precisely report the location and actual quantity of SVs in the genome.Conclusions: The quality of reference genomes and the density of selected nickases may relate to the accuracy of SV detection by BOM. Nucleotide-level validation is crucial to confirm and characterize SVs reported by optical mapping.
Data files
Genotype
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