Study : Early transcriptional response to Ralstonia solanacearum infection in the roots of tobacco (Nicotiana tabacum L.)

Identification

Name
Early transcriptional response to Ralstonia solanacearum infection in the roots of tobacco (Nicotiana tabacum L.)
Identifier
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Source
Description
This project was to study the genetic basis of bacterial wilt (BW) resistance in tobacco. A resistant variety D101 and a susceptible variety Changbohuang (CBH) were used in the experiment. Inoculation was performed by soaking roots of 30-days-old seedlings in a Ralstonia solanacearum suspension for 3 h. Mock inoculation (control) was performed using autoclaving-inactivated bacterial suspension. RNA was extracted from the seedling roots and sequenced on an Illumina HiSeq X Ten. Three biological replicates (samples) of each treatment were analyzed, each being a mixture of 6 seedlings. One replicate of D101-In was discarded due to poor RNA quality.

Genotype

Accession number Name Taxon