Study : ALBA protein complex reads genic R-loops to maintain genome stability in Arabidopsis

Identification

• Name: ALBA protein complex reads genic R-loops to maintain genome stability in Arabidopsis
• Identifier: dXJuOkVWQS9zdHVkeS9QUkpOQTQ5ODA5MA==
• Data link: Link to this study on EBI European Nucleotide Archive
• Description: The R-loop, composed of a DNA-RNA hybrid and the displaced single-stranded DNA, regulates diverse cellular processes. However, how cellular R-loops are sensed remain poorly understood. Here we report the discovery of the evolutionally conserved ALBA proteins (AtALBA12) functioning as the R-loops sensor of genic regions in higher plant Arabidopsis thaliana. AtALBA1 and 2 form a heterodimer protein complex and localize in the nucleus. While AtALBA1 binds to the DNA-RNA hybrid strand, AtALBA2 associates with single-stranded DNA in the R-loops. AtALBA12 preferentially binds to the genic R-loop regions that are associated with active epigenetic marks. Depletion of AtALBA1 or AtALBA2 results in hypersensitivity of plants to DNA damaging agents. Our results demonstrate the AtALBA12 protein complex is the Arabidopsis genic R-loops sensor for maintaining genome stability in Arabidopsis. Overall design: We conducted ssDRIP-seq with Arabidopsis wild-type (Col-0) and double mutant (alba1-1/alba2-1) seedlings to proflie the genowide R-loop levels.
• Data files: SRR8097478; SRR8097477