Study : Transcriptome-wide analysis of gene expression using detached first-pair rosette leaves before culture (time 0), at 10min to 12h after detachment Col-0, coi1-2 and sdg8-2 seedlings.

Identification

Name

Transcriptome-wide analysis of gene expression using detached first-pair rosette leaves before culture (time 0), at 10min to 12h after detachment Col-0, coi1-2 and sdg8-2 seedlings.

Identifier

dXJuOkVWQS9zdHVkeS9QUkpOQTQ5MzAyMQ==

Source

Data link

Link to this study on EBI European Nucleotide Archive

Description

To reveal the early molecular events upon wounding during DNRR from Arabidopsis leaf explants cultured on B5 medium without exogenous hormones, we carried out an RNA-seq experiment using leaf explants before culture (i.e. time 0), at 10 and 30 min after detachment, and at 1 to 12 h after detachment. The results showed that the upregulation of JA-related genes occurred as an early response to wounding (mainly within 1 h), prior to the upregulation of auxin-related genes (from 2 to 12 h). These results indicated that JA responds rapidly to wounding and earlier than does auxin. To analysis whether SDG8-mediated H3K36me3 is generally involved in JA-mediated gene upregulation in response to wounding. We evaluated changes in the transcriptome in sdg8-2 and coi1-2 leaf explants (2 h vs. time 0) by analyzing RNA-seq data, and compared them with transcriptomic changes in wild-type. Overall design: Arabidopsis seeds were germinated on ½ MS medium at 22°C under a 16-h light/8-h dark photoperiod. Detached leaf explants from 12-day-old seedlings were cultured on B5 medium without sucrose at 22°C under 24-h light conditions. Biological duplicates were performed for each timepoint analyzed.

Data files

Genotype

Accession number	Name	Taxon