Study : Arabidopsis thaliana Transcriptome or Gene expression


Arabidopsis thaliana Transcriptome or Gene expression
In this study, we systematically investigated the fate and phytotoxicity of carbon nanodots (C-dots, about 3 nm) in Arabidopsis thaliana (Arabidopsis), as well as the underlying potential mechanisms, by integrating transcriptomic, physiological and metabolomic techniques. The phytotoxicity of C-dots in Arabidopsis was concentration-dependent, C-dots at 125, 250, 500 and 1000 mg/L significantly reduced the root elongation by 25%, 40%, 56% and 61% compared with the control groups, but no significant inhibition were observed in 62.5 mg/L C-dots exposure. RNA-seq analyses showed that 76 and 715 genes in Arabidopsis roots while 112 and 636 genes in Arabidopsis shoots, which were involved in stimulus response, UDP-glycosyltransferase activity and cellular response to phosphate starvation, were up-regulated by 125 mg/L and 1000 mg/L C-dots, respectively. Meanwhile, 164 and 552 down-regulated genes in roots while 300 and 707 down-regulated genes in shoots were mainly involved in chloroplast structure and function.


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