Study : RNA-Seq of WT and -P treatment
Identification
Name
RNA-Seq of WT and -P treatment
Identifier
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Source
Description
To further explore the molecular mechanisms of P regulation in banana plants, we used RNA sequencing-based transcriptomic analysis for banana plants subjected to Pi deficit stress for 60 days.We detected 1900 significantly differentially expressed genes (DEGs) in aboveground plant parts and 7398 DEGs in root parts under low P stress. Gene ontology (GO) classification analysis showed that 156,291 GO terms belonging to molecular functions, 53,114 GO terms belonging to cellular components, and 228,544 GO terms belonging to biological processes were enriched in the aboveground and root components. A number of DEGs involved in energy metabolism-related processes, signal transduction, control of rhizosphere P activation, and Pi mobilization were found, which were confirmed by quantitative reverse-transcription PCR (RT-PCR) analysis. At the transcriptomic level, we detected 13 DEGs from different organs and with different functions in the time-course response to phosphorus deficiency stress. These DEGs may include some key genes that regulate the phosphorus network, increasing our understanding of the molecular mechanism of Pi homeostasis in banana. Overall design: Banana seedling roots and aboveground parts were harvested at 60 days after the start of experimental treatments, and these samples were then frozen immediately using liquid nitrogen and stored at −80 °C before being sent to BGI-Tech(Shenzhen, China) for RNA-seq by Illumina HiSeq2000.
Genotype
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