Study : Genome-wide occupancy of BDR1, BDR2 and FPA (ChIP-seq)
Genome-wide occupancy of BDR1, BDR2 and FPA (ChIP-seq)
We generated Arabidopsis lines expressing tagged (3X Myc tag) versions of BDR1 or BDR2 driven by their endogenous promoters on a bdrs triple mutant background (bdrs: cross of bdr1/AT5G25520 mutant SALK_142108C, bdr2/AT5G11430 mutant CS852350 and bdr3/AT2G25640 mutant SALK_059905). The genome-wide distribution of BDR1 and BDR2 in 8-day old seedlings were analyzed by ChIP-seq using an anti Myc antibody. We also analyzed by ChIP-seq the genome-wide distribution of FPA (AT2G43410) in wild-type seedlings using a rabbit anti-FPA polyclonal antibody raised against the C-terminal portion (position 536-901) of the FPA protein. Controls include sequencing of input DNA for each sample and a ChIP perfomed in wild-type seedlings with the anti-Myc tag antibody. We found that BDR1 and BDR2 are enriched at the border of a large number of genes in Arabidopsis genome and that FPA localizes mostly at the 3 end of genes. Overall design: ChIP-seq profiles in 8-day old seedlings of FPA, Myc tag-BDR1 and Myc tag-BDR2 (Myc tag-BDR proteins expressed in bdrs triple mutant background). Controls include sequencing of input DNA for each sample as well as a ChIP perfomed in wild-type plants (not expressing a tagged protein) with the anti-Myc tag antibody.