Study : Transcriptomic analysis of Verbena bonariensis leaves in response to low temperature
Transcriptomic analysis of Verbena bonariensis leaves in response to low temperature
The samples were respectively from three replicates of the cold-stress treatment and the control, then we obtained six cDNA libraries. 271,920 unigenes were produced from 406,641 assembled transcripts. Of these, 42,490 significantly differential expression genes (Corrected P-value <0.05) were obtained, including 20,412 up- and 23,078 down-regulated genes. Overall design: Verbena bonariensis seeds were available from Benary Manufacturer and grown in the greenhouse (16 h photoperiod, 25℃/16℃ day/night temperature). 3 months old seedlings were transferred to the following temperature treatment conditions: 4℃ for 24 h, followed by -4℃ for 4 h (wilting symptoms began to appear). Control seedlings remains unchanged. leaves mRNA profiles of the development were generated by deep sequencing, in triplicate, using Illumina HiSeq.