Study : Zea mays Epigenomics
Identification
Name
Zea mays Epigenomics
Identifier
dXJuOkVWQS9zdHVkeS9QUkpOQTMyNzU5Ng==
Source
Description
B73 seedling roots were pulse-labeled with the thymidine analog 5-Ethynyl-2-deoxyuridine (EdU) for 1 hour to allow actively replicating DNA to incorporate the EdU. The terminal 0-5 mm root segments were excised, fixed in 1% formaldehyde for 15 minutes, washed in PBS, and snap frozen. Nuclei were isolated from the frozen, excised roots, the incorporated EdU was clicked to Alexa Fluor-488 (AF-488), and total DNA was stained with DAPI. Nuclei were flow sorted based on EdU incorporation (AF-488 fluorescence) and DNA content (DAPI fluorescence) into various cell cycle populations. Chromatin extracted from sorted nuclei was used for ChIP and the resulting DNA Illumina sequenced.
Genotype
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