Study : RNA-seq of abscisic acid-reponsive transcription factors

Identification

Name

RNA-seq of abscisic acid-reponsive transcription factors

Identifier

dXJuOkVWQS9zdHVkeS9QUkpOQTMxOTMxOA==

Source

Description

Abscisic acid (ABA) is an essential hormone that allows plants to respond to environmental stresses such as high salinity, drought and cold. It also plays a pivotal role in seed maturation and germination. Because of its importance, transcriptome changes in response to ABA have been profiled extensively by the plant community. Very few ChIP-chip/seq of ABA-related TFs have been reported to date. To fill the knowledge gap about how ABA works at the transcriptional level, we carried out ChIP-seq on 21 TFs from 11 different families using both mock- and ABA-treated conditions. Analyses of the resulting 122 ChIP-seq datasets identified 326,698 TF binding events using a stringent statistical cutoff. Based on our data, a comprehensive regulatory network in Arabidopsis thaliana was constructed. We uncovered determinants of dynamic TF binding and defined a hierarchy among TFs to explain differential gene expression and pathway feedback regulation. By extrapolating regulatory characteristics observed for the canonical ABA pathway components, we identified a new family of transcriptional regulators modulating ABA and salt responsiveness, and demonstrate their utility to modulate plant resilience to osmotic stress. Overall design: Seedling were germinated and grown on nylon mesh in hydroponics for 3 days in dark before ABA or mock treatment. 10 uM ABA treatment or ethanol mock treatment for 1 to 60 hours.

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