Study : RNA-seq of abscisic acid-reponsive transcription factors [DIG]

Identification

Name

RNA-seq of abscisic acid-reponsive transcription factors [DIG]

Identifier

dXJuOkVWQS9zdHVkeS9QUkpOQTMxOTMxNg==

Source

Data link

Link to this study on EBI European Nucleotide Archive

Description

Abscisic acid (ABA) is an essential hormone that allows plants to respond to environmental stresses such as high salinity, drought and cold. It also plays a pivotal role in seed maturation and germination. Because of its importance, transcriptome changes in response to ABA have been profiled extensively by the plant community. Very few ChIP-chip/seq of ABA-related TFs have been reported to date. To fill the knowledge gap about how ABA works at the transcriptional level, we carried out ChIP-seq on 21 TFs from 11 different families using both mock- and ABA-treated conditions. Analyses of the resulting 122 ChIP-seq datasets identified 326,698 TF binding events using a stringent statistical cutoff. Based on our data, a comprehensive regulatory network in Arabidopsis thaliana was constructed. We uncovered determinants of dynamic TF binding and defined a hierarchy among TFs to explain differential gene expression and pathway feedback regulation. By extrapolating regulatory characteristics observed for the canonical ABA pathway components, we identified a new family of transcriptional regulators modulating ABA and salt responsiveness, and demonstrate their utility to modulate plant resilience to osmotic stress. Overall design: Three-day old etiolated seedlings were treated by 10 uM Dex for 4 hours in dark. Light-grown seedlings were grown on plates containing 500 nM Dex for 10 days.

Data files

Genotype

Accession number	Name	Taxon