Study : The second subunit of DNA-polymerase delta is required for genomic stability and epigenetic regulation

Identification

Name

The second subunit of DNA-polymerase delta is required for genomic stability and epigenetic regulation

Identifier

dXJuOkVWQS9zdHVkeS9QUkpOQTMxNTMwMg==

Source

Data link

Link to this study on EBI European Nucleotide Archive

Description

DNA polymerase δ plays crucial roles in DNA repair and replication, and maintaining genomic stability. However, the function of POLD2, the second small subunit of DNA polymerase δ, has not been characterized yet in Arabidopsis. During a genetic screen for release of TGS, we identified a mutation in POLD2. Whole-genome bisulfite sequencing indicated that POLD2 is not involved in the regulation of DNA methylation. POLD2 genetically interacts with ATR and DNA polymerase α. The pold2-1 mutant exhibits genomic instability with a high frequency of homologous recombination (HR). It also exhibits hypersensitivity to DNA-damaging reagents, and short telomere length. Whole-genome ChIP-seq and RNA-seq analyses suggest that pold2-1 changes H3K27me3 and H3K4me3 modifications, and these changes are correlated with the gene expression levels. Our study suggest that POLD2 is required for maintaining genome integrity, and properly establishing the epigenetic markers during DNA replication to modulate gene expression. Overall design: Examine DNA methylation, histone modification and RNA expression changes by whole-genome bisulfite sequencing; RNA sequencing; H3K27me3-, H3K4me3-, H3K9me2-, H3-ChIP-seq in A. thaliana wild type and pold2 mutant

Data files

Genotype

Accession number	Name	Taxon