Study : Arabidopsis thaliana cultivar:Col-0 Raw sequence reads
Identification
Name
Arabidopsis thaliana cultivar:Col-0 Raw sequence reads
Identifier
dXJuOkVWQS9zdHVkeS9QUkpOQTMxMTcyNQ==
Source
Description
The chloroplast-localized RNA helicase INCREASED SIZE EXCLUSION LIMIT2 (ISE2) is indispensable for proper plant development, and ise2 mutants are embryonically lethal. Importantly, loss of ISE2 in embryos is associated with increased intercellular trafficking that positively correlates with increased formation of plasmodesmata (PD). Intriguingly, phylogenetic analyses reveal that the ISE2 DEVH-type RNA helicase is conserved in all green photosynthetic organisms examined to date, suggesting that its functions are closely related to oxygenic photosynthesis. We show that ISE2 has the potential to interact with numerous chloroplast-localized proteins, and the interaction with the plastid ribosomal protein L15 (RPL15) was confirmed in vivo by co-immunoprecipitation. We also demonstrate that similar to ISE2, RPL15 is an essential gene, and depending on the approach plants depleted of RPL15 consistently develop chlorotic leaves or are embryonic lethal, as do ISE2-depleted plants. In addition, chloroplast-encoded proteins accumulate to lower levels in RPL15-depleted leaves, consistent with its role as a ribosomal protein. Some nucleus-encoded chloroplast proteins should little or no changes on loss of RPL15, suggesting that the effect on chloroplast proteins was specific. Notably, loss of RPL15 results in increased PD formation that positively correlates with increased intercellular trafficking in affected leaves. Collectively, these data support the emerging hypothesis that chloroplasts have an important role in regulating intercellular trafficking and PD biogenesis.
Genotype
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