Study : RNA-seq of Petunia floral buds for differences in flavonol-related genes

Identification

• Name: RNA-seq of Petunia floral buds for differences in flavonol-related genes
• Identifier: dXJuOkVWQS9zdHVkeS9QUkpOQTMwMDYxMw==
• Data link: Link to this study on EBI European Nucleotide Archive
• Description: This experiment was carried out to analyse the expression differences of genes involved in flavonol production between Petunia species. RNA was sequenced from intermediate stage buds (sepals and reproductive organs removed) of P. axillaris ssp. axillaris N, P. integrifolia ssp. inflata S6 (aka P. inflata S6), and the F1 (P. a. ssp. axillaris N x P. i. ssp. inflata S6) between the two species. Three biological replicates were sequenced per sample. For each biological replicate, six stage 4 buds were homogenized in liquid nitrogen and RNA extracted using the RNeasy Plant Mini Kit (Qiagen). RNA was sent to the Lausanne Genomic Technologies Facility (Lausanne, Switzerland) for sequencing. Cluster generation was performed with the sequencing libraries using the Illumina TruSeq PE Cluster Kit (v.3). Samples were single-end sequenced for 100 bp. The resulting sequences were used for differential expression analysis.
• Data files: SRR2890239; SRR2890258; SRR2890260; SRR2890261; SRR2890262; SRR2890263; SRR2890264; SRR2890266; SRR2890267