Study : Transcriptomic analysis of gibberellin-regulated genes and transcription factors in regulating bolting in lettuce
Identification
Name
Transcriptomic analysis of gibberellin-regulated genes and transcription factors in regulating bolting in lettuce
Identifier
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Source
Description
Lettuce prefers cool temperature, as high temperature will cause premature bolting, which seriously affects the lettuces’s eating quality and economic value. Thus, exploring the mechanism of bolting and preventing premature bolting is significant for agriculture. Prior to this study, we observed that there was a significant relationship between gibberellin and bolting in lettuce, but we did not explore it in depth. Thus, in this paper, the bolting-sensitive line S39 was selected for morphological, physiological, and transcriptomic comparisons at 37°C. From paraffin section results, we observed that S39 began bolting on the seventh day at 37°C. When bolting, GA3 and GA4 levels in the leaves and the IAA in the stem reached a maximum on the sixth day, and subsequently maintained the high contents. Additionally, when S39 began bolting on the fifth day, GA3 and GA4 increased and subsequently decreased, reaching a maximum on the fourth day in the leaves. Similarly, IAA contents reached a maximum in the stem on the fourth day. The control group did not bolt, as significant changes were not observed in GA3 and GA4 levels in the controls during the observed period. RNA-sequencing data revealed that transcription factors were implicated in regulating bolting in lettuce, suggesting that the high gibberellin contents in the leaves and IAA in the stem promoted bolting. Transcription factors may regulate expression and synthesis of related genes, such as hormones, which potentially regulate bolting in lettuce. Thus, our results lay a foundation for molecular breeding methods for desired planting periods in lettuce.
Genotype
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