Study : Genome-wide analysis of heat effect on rice transcriptome by RNA-seq

Identification

Name

Genome-wide analysis of heat effect on rice transcriptome by RNA-seq

Identifier

dXJuOkVWQS9zdHVkeS9QUkpOQTM5MjgxNg==

Source

Data link

Link to this study on EBI European Nucleotide Archive

Description

To impose the heat treatment similar to the Structure-seq experiment, we applied RNA-seq to 14-day-old rice (Oryza sativa) shoot tissue to investigate the effect of heat stress (42°C) as compared to control condition (22°C). Overall design: Two week old rice plants in pots were inverted and the shoots were immersed in a water bath at 22°C or 42°C for 10 min treatment, and the plants were then transferred to a growth chamber set at the same temperature as in the greenhouse (30°C) for ease of sampling during the recovery period (0 min, 10 min, 50 min, 1 h 50 min, 9 h 50min). Three rice shoots comprised one biological replicate, and two biological replicates were obtained for each treatment and time-point.

Data files

Genotype

Accession number	Name	Taxon