Study : Vitis vinifera transcriptome in response to Xylella fastidiosa

Identification

Name

Vitis vinifera transcriptome in response to Xylella fastidiosa

Identifier

dXJuOkVWQS9zdHVkeS9QUkpOQTM5MDY3MA==

Source

Data link

Link to this study on EBI European Nucleotide Archive

Description

<![CDATA[Examination of mRNA abundance profiles comparing 5 leaf samples of diseased grapevines (5 trees) to 5 healthy (non-infected) grapevines.Rationale: Pierce’s disease is a major threat to grapevines caused by the bacterium Xylella fastidiosa. Although devoid of a type 3 secretion system to deliver effectors inside host cells, this pathogen is able to influence host parenchymal cells from the xylem lumen by secreting a battery of hydrolytic enzymes. Defining the cellular and biochemical changes induced during disease can foster the development of novel therapeutic strategies.Methods: To this end we investigated the transcriptional, proteomic and metabolomic responses of diseased Vitis vinifera compared to healthy plants.Results: We found that several antioxidant strategies were induced, including the accumulation of GABA, arginine, proline and polyamine metabolism, as well as iron and copper chelation, but these were insufficient to protect the plant from chronic oxidative stress and resulting cell death. Notable upregulation of phytoalexins, pathogenesis-related proteins and various aromatic acid metabolites were part of the host responses observed. Moreover, upregulation of various cell wall modification enzymes followed the proliferation of the pathogen within xylem vessels, consistent with the intensive thickening of vessels’ secondary walls.

Data files

Genotype

Accession number	Name	Taxon