Study : Tobacco mosaic virus infection alters phloem associated translatomes in Arabidopsis thaliana

Identification

• Name: Tobacco mosaic virus infection alters phloem associated translatomes in Arabidopsis thaliana
• Identifier: dXJuOkVWQS9zdHVkeS9QUkpOQTM4MjU4MA==
• Data link: Link to this study on EBI European Nucleotide Archive
• Description: In this study we used vascular specific promoters and a translating ribosome affinity purification strategy to identify phloem-associated translatome responses to infection by tobacco mosaic virus (TMV) in the systemic host Arabidopsis thaliana ecotype Shahdara. Three different promoter:FLAG-RPL18 lines were used. These included two phloem specific promoters (pSUC2 and pSULTR2;2) as well as the more ubiquitously expressed cauliflower mosaic virus 35S promoter (p35S). Immunopurification of ribosome-mRNA complexes was accomplished by the method described in Reynoso et al. (Plant Functional Genomics: Methods and Protocols, 185-207; 2015). The dataset includes samples from the leaves of 5-week-old plants inoculated with TMV (1 mg/mL) or mock inoculated with sterile water. Overall design: 12 samples, 2 conditions (TMV or mock infected), 3 promoter lines, 2 biological replicates
• Data files: SRR5444354; SRR5444365; SRR5444364; SRR5444363; SRR5444362; SRR5444361; SRR5444360; SRR5444359; SRR5444358; SRR5444357; SRR5444356; SRR5444355; ERZ1173804; ERZ471093; ERZ526481; ERZ1173807; ERZ526478; ERZ1173806; ERZ526477; ERZ1173812; ERZ526476; ERZ1173798; ERZ526484; ERZ1173792; ERZ526485; ERZ1173808; ERZ471091; ERZ526479; ERZ1173797; ERZ526486; ERZ1173805; ERZ471092