Study : Structure-seq2: Sensitive and accurate genome-wide profiling of RNA structure in vivo
Identification
Name
Structure-seq2: Sensitive and accurate genome-wide profiling of RNA structure in vivo
Identifier
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Source
Description
We present Structure-seq2, which provides nucleotide-resolution RNA structural information in vivo and genome-wide. This optimized version of our original Structure-seq method increases sensitivity and data quality by minimizing formation of a deleterious by-product, reducing ligation bias, and improving read coverage. Structure-seq2 can employ a biotinylated nucleotide to facilitate the protocol. We have benchmarked Structure-seq2 on both mRNA and rRNA structure in rice (Oryza sativa) and apply Structure-seq2 to provide evidence of hidden breaks in chloroplast rRNA and a previously unreported N1-methyladenosine (m1A) in a nuclear-encoded rRNA. Overall design: 14 Structure-seq2 libraries are generated via MiSeq. Two replicates are included each of the standard Structure-seq2 protocol (both + and - DMS), and of the biotin variation.
Genotype
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